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Give the reason for passing bacterial smear through the flame before staining?
Wiki User
∙ 14y ago
It dries the smear and fixes the cells to the slide
Wiki User
∙ 16y ago
Wiki User
∙ 14y agoIf you don't heat fix the slide, the smear will wash off with the stain. The heat is kind of baking the smear onto the glass.
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Bacterial smears are fixed before staining to?
affix the cells to the slide and to kill bacteria
Do you apply waterproofing product before or after staining the deck?
After. If you waterproof before staining, the stain won't make it to the wood to stain it.
Why is the slide not heat fixed before negative staining?
First and foremost, the purpose of heat fixing is to drive stain into the bacterial cells, which in this case, you are staining the background, so there is not a need for heat fixing. Next, the process of heat fixing will shrink the cell by a little. This sorts of support the first reason as since there isn't the need to heat fix, then don't. By not heat-fixing, we actually see a more accurate morphology, arrangement and size of thr bacterial cell. Hope that my answers helps 😊
Why slide need to soak in alcohol before staining?
To remove unwanted microorganisms, dust particles and grease etc.....
What year did the first comet pass over the US?
Comets have been passing through the solar system since historical times. But no comet could have 'passed over' the US before 1776
Bacterial smears are fixed before staining to?
affix the cells to the slide and to kill bacteria
Can you heat fix blood smear before staining for bacterial examination?
You absolutely do not heat fix a blood smear before staining, that is, if you are looking at the blood cells. For bacteria, why wouldn't you culture it first and then heat fix, stain etc. I don't think heat fixing the blood stain would damage the bacterial cells so much as make it hard to differentiate the bacterial cells from the dead, shriveled, ruined blood cells, unless maybe you have like an electron microscope or something.
Do you apply waterproofing product before or after staining the deck?
After. If you waterproof before staining, the stain won't make it to the wood to stain it.
What is the importance of heat fixing the slide before staining?
You heat fix a slide by passing it through a blue flame a couple of times (with th cells facing up). you do this to denature any enzymes that might lyse the cells or interfere with the staining procedure. you also use it kill the organism and to adhere the organism to the slide for staining
What is the colour of onion peel before staining?
green
In capsule staining why you should not heat-fix the smear?
Capsular material is very moist (slimy) and any heating will cause it to shrink - it is for this reason that we will not heat fix the slide before staining. Also, heating may cause the bacterial cell to shrink resulting in a clear zone around the cell - which may cause cells which don't have capsules to appear as if they do.
What is the frequency of light passing through a ruby?
That depends entirely upon its frequency before it entered.
Why is the slide not heat fixed before negative staining?
First and foremost, the purpose of heat fixing is to drive stain into the bacterial cells, which in this case, you are staining the background, so there is not a need for heat fixing. Next, the process of heat fixing will shrink the cell by a little. This sorts of support the first reason as since there isn't the need to heat fix, then don't. By not heat-fixing, we actually see a more accurate morphology, arrangement and size of thr bacterial cell. Hope that my answers helps 😊
Do you use sanding sealer before or after staining?
Stain first, seal afterwards.
Why ruminantion is necessary for ruminanants?
short guts - so they need to predigest the grass before passing the food through the gut
Where does the American public have a voice in passing a bill through congress?
One way is through invited testimony before a Congressional committee. Another way is through written or verbal communication with your Congressman.
How did people prevent staining or rusting of knives before the invention of stainless steel?
Oil.
