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Capsular material is very moist (slimy) and any heating will cause it to shrink - it is for this reason that we will not heat fix the slide before staining. Also, heating may cause the bacterial cell to shrink resulting in a clear zone around the cell - which may cause cells which don't have capsules to appear as if they do.
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What is the purpose of staining the smear in malachite green during spore staining?
the purpose of boiling of smear in malachite green is to forces a stain to penetrate the endospore wall, it is necessary to heat the slide and the stain to prod the wall to allow the stain to enter.
In gram staining why will very thick smears give inaccurate results?
If a smear exhibits uneven thickness, overlapping cells may not get the proper exposure to the reagents. This results in uneven or mottled staining. For example, in the thicker areas of the smear, gram-negative cells may not decolorize sufficiently and end up staining purple.
What are the advantage and disadvantage of heat fixing?
adv..... heat fixing inactivates enzymes that would normally disrupt cell morphology and structure during staining and observation. dis....... if not done well much of the specimen can be destroyed in heat fixing.
What is the purpose of smearing in microbiology?
Smear are made for preparing slides for staining which are used in microscopy. The main purpose of smear is to seprate cluster of microbial cells so that we can see them seprately which is helpfull in studying there morphology, and arrangement in colony
Why do you air-dry a smear?
To avoid denaturing and destroying the smear.
What is the purpose of staining the smear in malachite green during spore staining?
the purpose of boiling of smear in malachite green is to forces a stain to penetrate the endospore wall, it is necessary to heat the slide and the stain to prod the wall to allow the stain to enter.
Why is it you should heat-fixing the air-dried before staining?
It is used to fix because to make the cell inactive or immoblie, but the main purpose is to fix the smear so that when we put stain and then flush it out with water ( or some time with alcohol) the smear should not wash out with dye.
In gram staining why will very thick smears give inaccurate results?
If a smear exhibits uneven thickness, overlapping cells may not get the proper exposure to the reagents. This results in uneven or mottled staining. For example, in the thicker areas of the smear, gram-negative cells may not decolorize sufficiently and end up staining purple.
What is gram variability?
If after staining, a smear reveals both purple(Gram +) and red cells(Gram -). This is generally an indication that the smear contains gram positive cells.
Give the reason for passing bacterial smear through the flame before staining?
It dries the smear and fixes the cells to the slide
What are the advantage and disadvantage of heat fixing?
adv..... heat fixing inactivates enzymes that would normally disrupt cell morphology and structure during staining and observation. dis....... if not done well much of the specimen can be destroyed in heat fixing.
During the performance of the simple staining procedure you failed to heat fix your EColi smear preparation Upon microscopic examination how would you expect this slide to differ from correct slide?
The bacterial smear will wash away during the staining procedure. This is avoided by heat fixation, during which the bacterial proteins are coagulated and fixed to the glass surface.
What happens if you don't heat fix a smear prior to staining of a bacterial slide?
smear will be washed( no smear will be left on the slide)
What is the purpose of smearing in microbiology?
Smear are made for preparing slides for staining which are used in microscopy. The main purpose of smear is to seprate cluster of microbial cells so that we can see them seprately which is helpfull in studying there morphology, and arrangement in colony
Function of inoculator loop?
Inoculating loops are for the transferring of microorganisms or for the staining of slides. ?æIt is also called a smear loop or a micro streaker.?æ
Why do you air-dry a smear?
To avoid denaturing and destroying the smear.
What is the preparation of smears and simple staining?
smear is the putting and fixing of staining sample on glass slide which is done by first putting the a drop of water on slide and then inoculation is put over it which is then spread slowly in round form by inoculating loop and dry it by very light heat to fix it.Simple staining is the process in which a dye knwon as methylene blue is spread over smear to colour the microbe whcih can be then washed by 70% alcohol so that extra dye can be removed and then the sample is ready to observe under microscope
