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Gel electrophoresis
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∙ 13y ago
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What does the charge of DNA have to do with DNA fingerprinting?
DNA is of a negative charge. So when gel electrophoresis is used on it the DNA fragments are attracted to the positive end of the electrophoresis. The fragments of different lengths travel down the gel towards this end. The longer length fragments travel less and so are farther from the positive end. By looking at these DNA fragments, which are created by cutting DNA with restriction enzymes one can compare and contrast DNA. Thus DNA fingerprinting can take place based on the different restriction sites in DNA (cut by the enzymes) forming different length segments of DNA.
What checks DNA for error?
DNA fingerprinting
What is the pattern of dark bands on photographic film that is made when DNA fragments are separated by gel electrophoresis and tagged?
DNA fingerprint
Process that restriction fragments of DNA are separated from each other by the use of electricity?
The process is referred to as gel electrophoresis. This is an analytical process where DNA fragments can be separated based on size within a gel under the influence of an electric field
Use of agrose in DNA isolation?
Agarose is not used in DNA isolation. Agarose is used to prepare a gel in which DNA fragments can be separated based on size
Analysis of fragments of DNA as a form of identification?
DNA Fingerprinting
Fragments of DNA as a form of identification?
DNA fingerprinting
What does the charge of DNA have to do with DNA fingerprinting?
DNA is of a negative charge. So when gel electrophoresis is used on it the DNA fragments are attracted to the positive end of the electrophoresis. The fragments of different lengths travel down the gel towards this end. The longer length fragments travel less and so are farther from the positive end. By looking at these DNA fragments, which are created by cutting DNA with restriction enzymes one can compare and contrast DNA. Thus DNA fingerprinting can take place based on the different restriction sites in DNA (cut by the enzymes) forming different length segments of DNA.
What checks DNA for error?
DNA fingerprinting
The use of RFLPs for genetic fingerprinting is based on?
The use of RFLPs in generic fingerprinting is based on the ability of restriction enzymes to dissect DNA into small fragments. There are many kinds of restriction enzymes made to cut various DNA sequences.
What physics principle involved in DNA fingerprinting?
The most obvious application of physics in DNA fingerprinting involves the separation of fragments of DNA based on their mass and charge. This is done in a "gel electrophoresis" apparatus that pulls the fragments through a gel using an electric field. The physics therefore is that of the electric field, electric charge, and the resulting force to mass ratio.
Difference between southern and northern blotting?
Southern Blotting refers to the identification of detailed sequences of DNA in which the DNA fragments are separated by electrophoresisNorthern Blotting refers to the identification of detailed sequences of RNA in which the RNA fragments are separated by electrophoresis
What is the pattern of dark bands on photographic film that is made when DNA fragments are separated by gel electrophoresis and tagged?
DNA fingerprint
Process that restriction fragments of DNA are separated from each other by the use of electricity?
The process is referred to as gel electrophoresis. This is an analytical process where DNA fragments can be separated based on size within a gel under the influence of an electric field
Use of agrose in DNA isolation?
Agarose is not used in DNA isolation. Agarose is used to prepare a gel in which DNA fragments can be separated based on size
What technique take advantage of repeating DNA sequences that do not code for proteins?
DNA fingerprinting
Explain how a DNA fingerprinting produced?
In DNA fingerprinting, enzymes are used to cut the DNA in the smaple found at the crime scence
