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What is the pattern of dark bands on photographic film that is made when DNA fragments are separated by gel electrophoresis and tagged?
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∙ 12y ago
DNA fingerprint
Wiki User
∙ 12y ago
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What is created by treating DNA with restriction enzymes to create fragments allowing them to travel in an agarose gel bed with electricity and capturing the results in a type of picture?
When DNA is treated with restriction enzymes, and the fragments are loaded onto a gel which is subjected to electrophoresis, we get a banding pattern of the DNA fragments with the farthest band (from the gel) of those fragments smallest in size.
Why does extracted DNA need to be fragmented?
In preparation for the electrophoresis step in "DNA fingerprinting" the electrophoresis process cannot separate meaningfully massive molecules like whole chromosomes. By using restriction enzymes that break the chromosomes at known places DNA fragments of a wide variety of lengths that the electrophoresis process can separate meaningfully will allow a pattern to be generated that can identify different individuals.
What do you sometimes call the resultant pattern of DNA fragments?
GENES
Which process involving DNA creates a DNA pattern or fingerprint unique to an individual person?
DNA fingerprinting
What is the process of analyzing the unique pattern of DNA fragments resulting from restriction enzyme cleavage called?
it is either gene splicing or genetic engineering.
A pattern produced from fragments of the DNA of a specific person?
Gel electrophoresis is the process that is used in DNA creates and pattern unique to an individual person. Each person has their own DNA patterns.
What is created by treating DNA with restriction enzymes to create fragments allowing them to travel in an agarose gel bed with electricity and capturing the results in a type of picture?
When DNA is treated with restriction enzymes, and the fragments are loaded onto a gel which is subjected to electrophoresis, we get a banding pattern of the DNA fragments with the farthest band (from the gel) of those fragments smallest in size.
Why does extracted DNA need to be fragmented?
In preparation for the electrophoresis step in "DNA fingerprinting" the electrophoresis process cannot separate meaningfully massive molecules like whole chromosomes. By using restriction enzymes that break the chromosomes at known places DNA fragments of a wide variety of lengths that the electrophoresis process can separate meaningfully will allow a pattern to be generated that can identify different individuals.
Following electrophoresis what is the resulting DNA pattern called?
footrprint
What do you sometimes call the resultant pattern of DNA fragments?
GENES
What is the pattern of dark bands on X-ray film made when an individuals DNA fragments are separated then probed and then exposed to an X-ray film?
Yeah I have no idea but I do know that you are rewriting answer from a Modern Biology worksheet because I am looking for the same thing
What is the purpose of ethidium bromide?
Ethidium bromide is an intercalating agent that attaches itself between the helix of a DNA. Because the ethidium molecule lights up when illuminated by an ultraviolet light, it is used often in biochemistry laboratories so that fragment of DNA that has been separated by gel can be visualized.
Which process involving DNA creates a DNA pattern or fingerprint unique to an individual person?
DNA fingerprinting
What kind of pattern of bands on an electrophoresis gel would show from the DNA samples taken the Dolly?
They would be the same since Dolly is clone.
A pattern of bands made up of specific DNA fragments is a?
DNA fingerprint
What is the effect of the run time on the observed pattern of restriction fragments?
the longer the run time the better the results?
How are DNA from hair follicle and skin cell used to determine if they belong to same person?
DNA can be extracted from tissue, in this case, epidermal layers of skin (keratin doesn't have nuclei) or the hair follicle. The DNA is then amplified using a process called polymerase chain reaction (PCR), to create many more copies of the DNA segments using special coding sequences called primers. The segments can then be digested with enzymes (restriction enzymes) to create DNA fragments. Differences in DNA sequences create different sized DNA fragments, which are then separated on a gel matrix using an electric current (electrophoresis). The pattern of different sized fragments is unique to an individual, somewhat like a bar code. Two similar fragment patterns are compared, and if similar suggest identity (RFLP - restriction fragment length polymorphisms). You increase the chances of proving identity between the hair and skin if many different restriction enzyme reactions are done showing similarity between many different fragment sizes.
