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One of the Conclusion of electrophoresis is Visualization of the DNA size. Second is Sequencing the length of DNA of the body.
electrophoresis takes segments of DNA that are already broken up and aligns them by length with an electric current. It doesn't cut the DNA.Added:No, they must be cut into smaller pieces by restriction enzymes ( HINDI, for instance ) before they are run in the gel.
Ethidium bromide and coomassie blue are some stains that can be used in DNA electrophoresis.
Because on the addition of the normal ddNTP to the sequencing, there will be addition of another nucleotide added by DNA polymerase which keeps DNA sequencing going.
PCR itself cannot be used to diagnose a disease; it is only useful for amplifying a DNA sample. Molecular analysis can only be done with subsequent techniques, such as electrophoresis and DNA sequencing.
Gel Electrophoresis
Electrophoresis is an analytical technique used to separate charged molecules based on the migration of molecules in an electric field. It is particularly useful in separating molecules such as: Deoxyribonucleic acid (DNA) Ribonucleic acid (RNA) ProteinsIt is commonly used as a diagnostic tool for detecting genetic mutations determining DNA sequencing and diagnosing certain diseases.
Gel electrophoresis
Gel Electrophoresis
Electrophoresis. Restriction enzymes are used to cut DNA into fragments. Solutions containing these fragments are placed on the surface of a gel to which an electric current is applied. The electric current causes the DNA fragments to move through the gel. Because smaller fragments move more quickly than larger ones, this process separates the fragments according to size.
Capillary electrophoresis is a technique used in laboratories to separate molecules based on their charge in order to study and analyze them. Capillary electrophoresis uses an electric charge to force the movement of molecules since each molecule will go a varying distance based on the weight of the molecule and their charge. Some areas of study that use capillary electrophoresis include DNA sequencing and pharmaceutical analysis.
Since the birth of DNA sequencing in the 70's several methods have been developed which have become increasingly more efficient. There are probably 10-15 mainstream ways of sequencing, although dye-terminator sequencing is the one primarily used