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The expression of a gene of interest can be ensured by combining it with a gene recessive to it.
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What are the two broad areas of use and two examples after a host cell grown in culture to form a clone of cells containing the cloned gene of interest?
Cloning and tissue culture .
What are expression vectors in recombinant DNA technology?
Expression vectors are plasmids used to produce (heterologous expression) proteins from your gene of interest in the expression host(such as E.coli, Yeast, Human cell lines). The gene of interest cloned in this vector (at the MCS) will be transformed in to the host for protein expression. check this out for more info:
A collection of host cells that house different cloned fragments of DNA?
gene library
What is in vitro mutagenesis and what does it help the scientist understand?
In vitro mutagenesis is a technique to discover the function of a gene by introducing specific changes into the sequence of a cloned gene, reinserting the mutated gene into a cell, and studying the phenotype of the mutant.
What purpose does restriction mapping serve when it comes to cloning genes?
The enzymes being used to cut the vector open allowing the insertion of the gene of interest. Once the gene has integrated into the host plasmid it can grown in a media conducive to which ever particular strain of bacteria it is. As the transformed bacteria grow in colonies each cell will possesses the gene that was inserted. Now there is a large quantity of "cloned" genes which can be isolated back out and examined. - Rough summary-
What was the name of the first cloned?
The first "cow" (it was actually a calf) in the world to be cloned was named Gene.
What is gene cloning and why do you need to clone a gene?
Gene cloning is the technique of recombinant DNA technology in which a desired gene of interest having a striking characteristic feature is cloned. The gene may be selected because it appears to influence the organism in a striking manner, or to determine the role of the gene in the organism. Genes can be clones for industrial purposes, for instance the production of vaccines and insulin, or for research purposes, to determine what the role of the gene is. Gene cloning requires a basic knowledge of the gene's sequence, or flanking sequences. Genes can be cloned using polymerase chain reaction (PCR), if the sequence is known, or by cutting genomic DNA with restriction enzymes (to create smaller chunks of DNA). Usually, once a fragment containing gene has been identified using restriction enzymes, it is sequenced and PCR is used to isolate the specific sequence within the fragment.
What are the two broad areas of use and two examples after a host cell grown in culture to form a clone of cells containing the cloned gene of interest?
Cloning and tissue culture .
What are expression vectors in recombinant DNA technology?
Expression vectors are plasmids used to produce (heterologous expression) proteins from your gene of interest in the expression host(such as E.coli, Yeast, Human cell lines). The gene of interest cloned in this vector (at the MCS) will be transformed in to the host for protein expression. check this out for more info:
How do you predict a PCR product size from a gene cloned into an expression vector?
It all depends on where you primers are. Presumably you will have one primer that sits on the cloned gene and one that sits on the vector (that way you only get a product if the gene has cloned successfully). As long as you know where your primers land, it should be easy to work out how big the PCR product will be simply by adding the distance from the primer on the gene to the end of the gene and the distance from the primer on the vector to the end of the vector.
A single isolated gene can be cloned MOST rapidly using genetically engineered?
bacteria
A collection of host cells that house different cloned fragments of DNA?
gene library
Who identified the p53 gene?
The p53 gene was identified in 1979 by Arnold Levine, David Lane, and William Old. It wasn't until 1989 that the gene was found to be a tumor suppressor.
How were the first cow cloned?
The information is unknown how the first cloned cow was made. The first known cloned cow was named Gene and was cloned on February 7, 1997.
Antibiotic in gene cloning?
If antibiotic resistance is added to the gene being cloned, antibiotics can be used to isolate the transformed bacteria (ones with the gene being cloned) by killing off all non-transformed bacteria, that don't have the antibiotic resistance. There is a chance that the non-transformed bacteria can mutate to develop antibiotic resistance.
Why is it easier to perform a test for huntington's disease now that the gene has been cloned?
we can map and sequence it
How can you prove that gene cloning is complete?
You can prove gene closing is complete by comparing the cloned genes to the original. If any differences are present, the cloning process was not successful.
