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Expression vectors are plasmids used to produce (heterologous expression) proteins from your gene of interest in the expression host(such as E.coli, Yeast, Human cell lines). The gene of interest cloned in this vector (at the MCS) will be transformed in to the host for protein expression. check this out for more info:
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Describe one similarity between PCR and recombinant DNA technology.?
PCR is the abbreviation for polymerase chain reaction. It is similar to recombinant DNA technology in that both have the ability to sequence DNA.
Why cleavage of DNA is done in recombinant DNA technology?
Recombinant DNA technology is the most emerging technique for the production of DNA for the useful bio-materials like insulin. So to produce recombinant DNA two different DNA is rejoined. so cleavage is done to extract the desired DNA and then joined again.
What is a recombinant enzyme?
Enzymes derived from recombinant DNA technology as opposed to naturally occurring enzymes
Splicing DNA from two different organisms produces a new DNA segment called?
When DNA contains parts from two or more organisms it is recombined. Recombinant DNA is often used in genetic engineering. A natural process of DNA recombination is called sexual reproduction.
What does plasmids do?
plasmids are vectors that are used to replicate recombinant DNA in a host organism or cell. they are normally used in cloning experiments.
What are the requirements for recombinant DNA technology?
gene of interest 2.molecular scissor 3.molecular carrier or vector 4.expression system .These are the requirments for recombinant DNA technology.
Significance of vector in DNA recombinant technology?
Vector are plasmid DNA, act as a molecular vehicles to carry genes or DNA of interest. In rDNA technology vectors used to clone the gene by ligation. This chimeric DNA or plasmid can be propagated in E.coli as the vector carries its own origin of replication. Expression plasmid vectors can be used to produce proteins from the gene of interest.
What is puc vectors?
A pUC vector a circular, double stranded DNA molecule normally used for recombinant protein expression. It is not a math vector.
What is used to make many copies of DNA?
Recombinant DNA technology PCR
What used to copy DNA?
Recombinant DNA technology
What is the substance required to cleave the vector DNA during recombinant DNA technology?
A restriction enzyme, also called a restriction endonuclease, is needed to cleave vector DNA when using recombinant DNA technology.
Describe one similarity between PCR and recombinant DNA technology.?
PCR is the abbreviation for polymerase chain reaction. It is similar to recombinant DNA technology in that both have the ability to sequence DNA.
Why cleavage of DNA is done in recombinant DNA technology?
Recombinant DNA technology is the most emerging technique for the production of DNA for the useful bio-materials like insulin. So to produce recombinant DNA two different DNA is rejoined. so cleavage is done to extract the desired DNA and then joined again.
What is a recombinant enzyme?
Enzymes derived from recombinant DNA technology as opposed to naturally occurring enzymes
Why might retroviral vectors for gene therapy increase the patient's risk of developing cancer?
They might integrate recombinant DNA into the genome in ways that misregulate the expression of genes at or near the site of integration
What genetic technology is primarily responsible for the genetic technology revolution?
recombinant DNA
How can recombinant DNA technology be used to combat HIV infections?
How can recombinant DNA technology be used to combat HIV infections?
