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The restriction enzymes have ability to cut on a specific sequences that are knwon as palandreomic sequences now they cut in two way i.e. blunt and sticky for e.g. if we have a sequece of ATTCGTAAGC it will be cutted in two way as ...................................................TAAGCATTCG 1. ATTCG......... TAAGC i.e., blunt or straight form all ends ....TAAGC .........ATTCG 2. ATT .................CGTAAGC ....TAAGCAT .................TCG i.e., sticky or uneven ends The reason for this phenomena is still not clear or knwon
The sticky ends generated by restriction enzymes can easily be joined using an enzyme called ligase. Blunt ends however, cannot be joined so easily. This is why restiction enzymes that create sticky ends are more useful. If blunt ends result, small segments called modifiers are attached to the sticky ends. These modifiers are nucleotide sequences that have sticky ends and attach to the blunt ends, thus making them sticky ends.
They are called "sticky ends".
can join with each other.
Restriction Enzyme
sticky ends are more specific and easier to ligate
The restriction enzymes have ability to cut on a specific sequences that are knwon as palandreomic sequences now they cut in two way i.e. blunt and sticky for e.g. if we have a sequece of ATTCGTAAGC it will be cutted in two way as ...................................................TAAGCATTCG 1. ATTCG......... TAAGC i.e., blunt or straight form all ends ....TAAGC .........ATTCG 2. ATT .................CGTAAGC ....TAAGCAT .................TCG i.e., sticky or uneven ends The reason for this phenomena is still not clear or knwon
Restriction enzymes can leave blunt or sticky ends. This will depend on where they cut the DNA, if they cut at the same point on both strands, they will leave a blunt end, if they cut at different points, they will leave sticky ends. For example: (| represents point of cut) Blunt end ATC|GCTA TAG|CGAT Sticky end A|TCGCTA TAGCGA|T
The sticky ends generated by restriction enzymes can easily be joined using an enzyme called ligase. Blunt ends however, cannot be joined so easily. This is why restiction enzymes that create sticky ends are more useful. If blunt ends result, small segments called modifiers are attached to the sticky ends. These modifiers are nucleotide sequences that have sticky ends and attach to the blunt ends, thus making them sticky ends.
These sticky ends, if they two pieces match, they will join together to form a recombinant DNA.
the enzymes cause sticky ends to form------------------------------------------------the question is WHAT ARE STICKY ENDS, not how are sticky ends formed.the answer is "single stranded pieces of DNA left at the ends of restriction fragmants"-simone :)The actual answer is: single-stranded ends of fragments of double-stranded DNA
A Sticky End, referring to Biology is recombinant DNA. After DNA has been cut by a restriction enzyme it has "sticky ends" or recombinant DNA at the ends.
In general, sticky end cloning and blunt end cloning
They are called "sticky ends".
Generally, cDNA's do not have sticky ends - also known as overhangs. However, if the research requires it, they can be engineered to have overhangs
HaeIII
Sticky ends are produced by cutting the DNA in a staggered manner within the recognition site producing single-stranded DNA ends. These ends have identical nucleotide sequence and are sticky because they can hydrogen-bond to complementary tails of other DNA fragments cut by the same restriction enzyme.