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Restriction Enzyme
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∙ 11y ago
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Fragments of DNA that have complementary sticky ends?
can join with each other.
How does the order of the nitrogenous bases determine the type of protein that will be produced?
Every 3 bases specifies either an amino acid or a terminator. The amino acid sequence creates the protein. The terminator ends the protein.
What are unpaired ends of DNA called?
They are called "sticky ends".
What are sticky tails?
when the restriction enzymes cut off the ends of the DNA, they become "sticky" so that they are easily glued back together
A restriction enzyme is likely to cut which kind of molecules?
DNA molecules. A strand of DNA molecules can be cut to have blunted ends or jagged ends (sticky ends).
Fragments of DNA that have complementary sticky ends?
can join with each other.
What are stick ends?
the enzymes cause sticky ends to form------------------------------------------------the question is WHAT ARE STICKY ENDS, not how are sticky ends formed.the answer is "single stranded pieces of DNA left at the ends of restriction fragmants"-simone :)The actual answer is: single-stranded ends of fragments of double-stranded DNA
What does the term sticky ends refer to in gene splicing?
Sticky ends are produced by cutting the DNA in a staggered manner within the recognition site producing single-stranded DNA ends. These ends have identical nucleotide sequence and are sticky because they can hydrogen-bond to complementary tails of other DNA fragments cut by the same restriction enzyme.
What seals the sticky ends of restriction fragments to make recombinant DNA?
These sticky ends, if they two pieces match, they will join together to form a recombinant DNA.
When a restriction enzyme cuts a dna molecule the cuts are staggered so that the dna fragments have single stranded ends why is this is important in recombinant dna work?
It is important because the fragments will bond to other fragments with complementary single-stranded ends.
What is a sticky end?
A Sticky End, referring to Biology is recombinant DNA. After DNA has been cut by a restriction enzyme it has "sticky ends" or recombinant DNA at the ends.
How are the cleaved DNA fragments from two sources able to recombine?
Sometimes, when the cleaved DNA fragments both have sticky ends, they naturally anneal due to complementary base pairing. However, an enzyme called DNA Ligase is used for joining cut strands of DNA together. There is a ligase called t4 Ligase that joins blunt ends because it is stronger.
What are unpaired ends of DNA called?
They are called "sticky ends".
How does the order of the nitrogenous bases determine the type of protein that will be produced?
Every 3 bases specifies either an amino acid or a terminator. The amino acid sequence creates the protein. The terminator ends the protein.
Do cdnas have sticky ends?
Generally, cDNA's do not have sticky ends - also known as overhangs. However, if the research requires it, they can be engineered to have overhangs
Which enzymes does not make sticky ends?
HaeIII
Why there are sticky and blunt end produced in DNA?
sticky ends are more specific and easier to ligate
