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In 1619, Rolfe married Jane Pierce. They had a daughter, Elizabeth, in 1620.

Rolfe died in 1622 after his plantation was destroyed in an Indian attack. It remains unclear whether Rolfe died in the Indian massacre or whether he died as a result of illness.

Rolfe's son Thomas after being educated in England, returned to Virginia, where he married. It has been said that his wife's name was Jane Poythress. However, this has been disputed.

Thomas Rolfe and his wife had one child, Jane, who married Robert Bolling in 1675. She died in 1676 leaving one son, John, born the same year.

So all in all, he died of an illness.

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