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How do you calculate concentration of NaCl from absorbance value (only value given)?
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How do you find the concentration of potato cells immersed in salt solutions?
You would have to use a spectrophotometer to measure the absorbance of your unknown solution. But first, you need to make several solutions with known concentrations. Measure the absorbance of the known concentrations and plot them on an X and Y axis where X equals concentration and Y equals absorbance. Do a best-fit line for your data. Measure the absorbance of your unknown solution. Find this value on your Y-axis and find out where this value intersects with your line of best fit. The X value at the intersection is your concentration of potato cells. By the way, make sure you use the same wavelength throughout the experiment.
Would the concentration of a diluted protein shake with an absorbance value of 70mg ml be darker than a protein concentration of 15mg ml?
It would be darker... As the concentration gets stronger, it gets darker.
What is external calibration and is it the same as the manual calibration of equipment?
Well, external calibration is a method used in analytical chemistry to determine the concentration of an unknown analyte. In essence, you take known concentrations of the analyte and plot it on an absorbance or transmittance graph to get a linear plot. And then you take that linear equation and plug in the absorbance or transmittance value received from the unknown solution and get the concentration. An example of this is if you want to find out the amount of calcium in a vitamin tablet. Dissolve the vitamin tablet and test the solution to get an absorbance value. Then test by the same method various concentrations of a calcium solution, plot this on a graph of absorbance vs. concentration and there yah go.
Why do value of absorbance have no units?
Because it's a relative value.
Why are the absorbance values obtained from the conversion chart rather than the absorbance scale on the spectronic 20?
because that chart gives a more accurate value than the absorbance scale on the specthometor
True or false As the concentration of a colored solution increases the absorbance value decreases?
Absorbance rises with concentration because there is more material for a given frequency of light to be absorbed in. Your statement is false.
In a plot of absorbance vs concentration what does the slope represent?
The slope of absorbance vs concentration reptresents the value of εb, where ε is the absorbtivity with units of (L/mol cm) and b is path length measured in cm.
How do you find the concentration of potato cells immersed in salt solutions?
You would have to use a spectrophotometer to measure the absorbance of your unknown solution. But first, you need to make several solutions with known concentrations. Measure the absorbance of the known concentrations and plot them on an X and Y axis where X equals concentration and Y equals absorbance. Do a best-fit line for your data. Measure the absorbance of your unknown solution. Find this value on your Y-axis and find out where this value intersects with your line of best fit. The X value at the intersection is your concentration of potato cells. By the way, make sure you use the same wavelength throughout the experiment.
Would the concentration of a diluted protein shake with an absorbance value of 70mg ml be darker than a protein concentration of 15mg ml?
It would be darker... As the concentration gets stronger, it gets darker.
How do you calculate concentration of phosphoric acid?
You can do it with a pH probe, or, if you are given the Ka value for phosphoric acid, you can do some math and figure it out.
What is external calibration and is it the same as the manual calibration of equipment?
Well, external calibration is a method used in analytical chemistry to determine the concentration of an unknown analyte. In essence, you take known concentrations of the analyte and plot it on an absorbance or transmittance graph to get a linear plot. And then you take that linear equation and plug in the absorbance or transmittance value received from the unknown solution and get the concentration. An example of this is if you want to find out the amount of calcium in a vitamin tablet. Dissolve the vitamin tablet and test the solution to get an absorbance value. Then test by the same method various concentrations of a calcium solution, plot this on a graph of absorbance vs. concentration and there yah go.
Why should an absorbance level not exceed a value of 1?
Since the absorbance level is actually just a fraction of radiation absorbed at a given wavelength, it should never rise above %100.
Which formula do you use for the calculation of absorptivity?
ANSWER:"e is a measure of the amount of light absorbed per unit concentration".Molar absorbtivity is a constant for a particular substance, so if the concentration of the solution is halved so is the absorbance, which is exactly what you would expect.The formula for the molar absorptivity is given as followings:A=ecle=A/cle = the molar absorptivitywhere A is known as the A is known as the absorbance, l measures the length of the solution the light passes through,c is theconcentration of solution in mol /dm^3.Remember that the absorbance of a solution will vary as the concentration or the size of the container varies. Molar absorptivity compensates for this by dividing by both the concentration and the length of the solution that the light passes through. Essentially, it works out a value for what the absorbance would be under a standard set of conditions - the light travelling 1 cm through a solution of 1 mol dm-3. a
Why do value of absorbance have no units?
Because it's a relative value.
Calculate concentration of hydroxide ion with given pH?
Ist step, calculate pOH value by using formula pH + pOH = 14 2nd step, pOH = -log[OH], [OH] = - Antilog of pOH
For a given hfe calculate hfc in electronic circuits?
how to calculate the value of hfc for a given hfe
Why are the absorbance values obtained from the conversion chart rather than the absorbance scale on the spectronic 20?
because that chart gives a more accurate value than the absorbance scale on the specthometor
