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HPLC purity :
It explains how pure our analyte is in the given mixture .It is not related to the how much our analyte is in the given mixture.
i.e Percentage of a our analyte with out impuritys in HPLC.(Known or Unknown)
HPLC assay :
It explains how much is our analyte in the given mixture(The content of our component in the given mixture).It is not related to analyte purity.
HPLC potency :
It is measurement of our analyte how potent it is.
i.e Purity of our analyte with out all possible impuritys like chromatographic impuritys(HPLC,GC-Residual solvents,TLC),heavy metals,sulphated ash ..etc
For example:
If we have a analyte of some X of purity 99.5%.
Prepare 20%,60% and 90% of solution of X.inject all these solution in hplc.
For 20% solution you will get 99.5% purity and 20% assay.
For 60% solution you will get 99.5% purity and 60% assay
For 90% solution you will get 99.5% purity and 90% assay.
Wiki User
∙ 12y ago
Wiki User
∙ 10y agoHpic purity can be determined by chromatography only. Hpic assay can be calculated by chemical. This is necessary for quality control in drug manufacturing for safety and effectiveness.
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What is assay by HPLC?
HPLC is high-performance liquid chromatography. This method is used for the separation and production of chemicals but also for chemical analysis.
What is the difference between potency and assay?
Potency is the purity of the compound ;this states % available of the main component without water and residual solvents Assay is defined as Potency plus water and residual solvents and other remaining fractions please note assay can also be expressed as as is basis or dry basis Assay and Potency are same if assay is exprtessed as as is basis please note Potency word is mainly used for Antibiotics and bilogicals courtsey ; CDER , USFDA ; Dr.Uttam Krishna
What is single point threshold in peak purity of hplc?
Single point threshold is show only peak maxima spectra whenever peak purity index is shows the front, tail and peak maxima spectra. for pure peak we have to consider three point peak purity which shows that your purity angle should be less than purity threshold which is clearly shows that your peak is specrally pure and homogeneous.
What factor used in percent Purity of sodium hypochlorite?
When we will calculate purity of sodium hypochlorite factor is 74.441
How do you distinguised np-hplc and rp-hplc?
NP-HPLC is "Normal Phase" HPLC, wherein the solvents used are less polar than the substrate in the HPLC column (e.g. using hexane or dichloromethane with a silica HPLC column). RP-HPLC is "Reverse-Phase" HPLC, wherein the solvents used are more polar than the substrate in the HPLC column (e.g. using Water and Methanol with a octadecylsilane (ODS or C18) column).
What is assay by HPLC?
HPLC is high-performance liquid chromatography. This method is used for the separation and production of chemicals but also for chemical analysis.
What is the formula to calculate assay?
sample area/standard area*standard weight/sample weight*standard purity/100*100
What is the test that determines the amount and purity of a given chemical in a preparation in the laboratory?
Assay
How you calculate rrf value in hplc methods?
Yes.
What is rs-hplc?
"RS-HPLC method" means "Related Substance HPLC Method".
Why assay by hplc limit is 98 percent to 102 percent?
If the substance under test is purer than the standard, the assay comes more than 100 pct.
How do you calculate the assay percent in chemicals?
4.4 g of NaOH
Peak-to-valley ratio in HPLC?
to calculate signal to noise use this formulae peak hight *2/noise level
What is an assayers office?
Assay offices are institutions set up to assay (test the purity of) items, to protect consumers. Upon successful completion of the assay, (i.e. the metallurgical content is found to be equal or better than that claimed by the maker and it otherwise conforms to the prevailing law) the assay offices typically stamp a hallmark, punze (G.) or poinçon (F.) on the precious metal item to certify its metallurgical content
What is single point threshold in peak purity of hplc?
Single point threshold is show only peak maxima spectra whenever peak purity index is shows the front, tail and peak maxima spectra. for pure peak we have to consider three point peak purity which shows that your purity angle should be less than purity threshold which is clearly shows that your peak is specrally pure and homogeneous.
What is the difference between potency and assay?
Potency is the purity of the compound ;this states % available of the main component without water and residual solvents Assay is defined as Potency plus water and residual solvents and other remaining fractions please note assay can also be expressed as as is basis or dry basis Assay and Potency are same if assay is exprtessed as as is basis please note Potency word is mainly used for Antibiotics and bilogicals courtsey ; CDER , USFDA ; Dr.Uttam Krishna
What factor used in percent Purity of sodium hypochlorite?
When we will calculate purity of sodium hypochlorite factor is 74.441
