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First off, there are many bioinformatics techniques, but in terms of experimental methods, there are a few methods:

a) 5' RACE PCR aka Primer Extension assay (using radiolabeled oligonucleotide primers)

b) Site directed mutagenesis of promoter region (starting downstream of the -10 region) and In vitro transcription assay

c) Deep cap analysis gene expression (CAGE) d) S1 Nuclease Mapping

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Q: How do you determine the transcription start site?
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