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CONVERT TO THE MOLE (0.075 m/l) * (157.67mw) =11.82 g/l

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Q: How do you make up 75mM Tris HCL buffer solution?
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How do you prepare 20 mM tris hcl buffer pH 7.4?

to prepare 100ml of 100mM Trissolution: Mol wt of Tris=121.14121.14g in 1000ml ----> 1M12.11g in 100ml -------->1M1M=1000mM121.1g---->1000mM12.11g ----------->100mM1.211g in 100ml and 100mM Tris


Can be mixed in a solution with H2CO3 to make a buffer?

H2CO3 is not used as buffer.


What can be mixed in solution with H2CO3 to make a buffer?

H2CO3 is not used as buffer.


How would you make 1l using 10mm tris hcl 50mm kcl 1.5mm mgcl2 ph8 3 25 degreesc?

Need calculation on how to make this buffer


How do you make buffer solution pH10?

See the link below.


How do you make 1m kh2po4 buffer?

Make Solution A by dissolving 174.18g of K2HPO4 in 1L of dH2O. Make solution B by dissolving 136g of KH2PO4 in 1L of dH2O. now mix solution A and B and finally adjust pH of your buffer.


What Volume of 5M HCl to make tris buffer at pH 7.5?

The formula weight is 121.5 --> this is equivalent to 1M with 121.5g tris in 1L dH20. For a 5M stock, use 5x as much tris in the same 1L dh20.607.5 g tris into 800ml dH2O - stirring - then pH to 7.5 with 6M HCl and QS to your final volume of 1L


How do you make this solution?

I don't know how to make the solution below. Low salt buffer: 10 mM phosphate buffer, 10 mM NaCl, pH 7.4. Could you tell me the method in detail?


Why a buffer is used and the effects of a buffer on a solution?

A buffer is used to resist the change in pH when acid or alkali is added to a solution. This makes it a stable environment, eg. for enzymes. The buffer stops the pH of the solution changing too drastically.


How do you make 1X TE buffer?

10 mM Tris pH 7.5 and 1mM EDTA pH 8.0 For 1 L : 10 mL of 1M Tris-Cl pH 7.5 and 2 mL of 500mM EDTA pH 8.0


Sodium acetate buffer?

can I make sodium acetate buffer 0.2M Ph=5 whit sodium acetate buffer 3m Ph=5 solution?


Can vinegar be used to make a buffer solution?

Vinegar (acetic acid) is a weak acid, so yes it can be used to make a buffer.