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Q: How do you separate DMSO layer and water?
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Why is DMSO used as a control?

Many test compounds (drugs, inhibitors, etc) are not soluble in water and therefore dimethylsulfoxide (DMSO) is used as a solvent instead. The compound dissolved in DMSO is what is used to treat the cell or animal and therefore you must prove that it is the compound, not the DMSO which is causing any results seen. To ensure this, you have a control that contains only DMSO and not the test compound. This is often referred to as a "vehicle control".


When oil and water are mixed two separate layers form. Each layer is called a what?

Each layer is a phase.


How do you dilute cyclosporine?

In organic solvents (DMSO, ethanol, chloroform, methylene chloride). The most common solvent used to dissolve cyclosporine is DMSO. Water will not work very well.


Are there more polar solvent than water for biology work?

yes DMF, DMSO..


What can be done to separate oil from water?

Wait. Oil will float on water. Siphon off the top layer after seperation


How do you separate gasoline and water where gasoline forms the upper layer in the mixture?

you can use an instrument called separating funnel to separate this mixture.


Water and chloroform are mixedthe components separate into two layerseach of which smells strongly of chloroformHow would you quickly and easily confirm which layer is water and which is chloroform?

Always the bottom layer will be of chloroform and upper layer of water due to difference in the density.


What action occurs when oil and water is mixed in a separation funnel?

The oil and water will separate into two layers with oil as the top layer.


What is the difference betwen DMSO and DMSO-d6 solution?

One difference is that DMSO-d6 is going to cost more. Chemically speaking, the diffference is that the hydrogens in the -d6 variety have been replaced by deuterium.


How TO remove DMSO?

1. If the compound is soluble in diethyl ether, water wash will completely remove DMSO from the ethereal solution . 2. Otherwise, add little ethanol/ water to the sample, then freeze it at -50 degrees Celsius and lypolise it under high vacuum condition (0.01mm Hg or below).


Is the ozone layer considered a separate layer of the atmosphere?

No it is not. It is there in stratosphere.


Can you separate water and Aniline using separating funnel?

At 20o C aniline's rate of solubility in water is 3.6g/mL. So, aniline mixes with water unlike kerosene which forms a layer above the water. Separating funnel is used to separate two immiscible liquids and cannot separate miscible liquids. Hence, we cannot separate water and aniline using separating funnel.