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1)Human immunodeficiencyvirus contains an RNA as its genetic material.Between standard PCR and RT PCR techniques, which one would you employ to amplify a desired gene from this RNA virus.Explain how you would monitor disease progression and therapy?
RT PCR Test is the most accurate test while pcr test or rapid test can get you results very quickly, the results may not always be accurate.
Why test for menstrual blood for TB PCR is done?
TB PCR blood test is done for rapid detection of Tuberculosis. This has proven to be the fastest and most effective detection method.
How will you detect the presence of casein?
With ELISA test or other allergen test like pcr or atp.
What is the use of maintaining a certain pH in pcr?
PCR is an enzymatically guided process. In optimum pH the enzyme will work best.
What are the different types of polymerase chain reaction techniques?
types of pcr: AFLP -PCR. Allele-specific PCR. Alu-PCR. Assembly -PCR. Assemetric -PCR. Colony -PCR. Helicase dependent amplification. Hot start pCR. Inverse -PCR. Insitu -pCR. ISSR-PCR. RT-PCR(REVERSE TARNSCRIPTASE). REAL TIME -PCR
What is a PCR test used to identify?
A PCR test amplifies a single or few copies of DNA and creates potentially thousands or millions of copies. The most common reasons are for cloning, diagnosis of hereditary disease, genetic fingerprints, and analysis of genes.
What does pcr rely on to work for applications like crime scenes?
pcr rilies on the ability of dna- copying enzymes to remain stable at high temperature
What is pcr and types of pcr?
PCR is a biotechnological method to amplify your gene (DNA) of your interest. It produce millions of your DNA fragments hence used in cloning. There are variants of this method using the same thermocycling principle such as touch down PCR, gradient PCR, RFLP, multiplex PCR, Q PCR, RT PCR and so on.
How do you use a Southwell Plot to estimate the critical load and initial imperfection of a column?
The Southwell Plot method enables you to test a column in a non-destructive manner so that its buckling behaviour can be determined. If we look at the Southwell Plot equation: P/y = 1/Pcr * y + a/Pcr Here we have: P = Applied load y = mid-height displacement Pcr = Critical buckling load a = initial mid-height deformation (or imperfection as you called it) Using test data, you can plot a graph with P/y along the y-axis and y along the x-axis. This will give you a slope of 1/Pcr and an intercept at y=0 of a/Pcr. From there the value of 'Pcr' and 'a' can be determined. It is interesting to see how the slope of this curve is often very linear, especially for elastic buckling cases. It is for this reason that you do not need to test a column to failure.
What is the use of dNTP?
The use of dNTP is PCR and multiplex PCR
PCR stands for?
PCR stands for Polymerase Chain Reaction.
What is the purpose of a PCR machine?
A PCR machine also known as a thermal cycler is a machine used to amplify segments of dna via the PCR which stands for polymerase chain reaction. PCR machines may also be used to test temperature sensitive reactions. The first step of the machine is to heat the samples to 94-96 degrees, then the temperature is lowered to 50-65 degrees, then the mixtures temperature is raised to 72 degrees to synthesize a new dna strand.
