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Ethidium bromide binds with DNA and slips in between its hydrophobic base pairs and stretches the DNA fragment, removing water molecules from the ethidium cation. The result of this dehydrogenation is an increase in fluorescence of the ethidium (as well as the cell).
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What does ethidium bromide stain in a cell?
Ethidium bromide is a chemical that is able to intercalate (fit between the bases) between the bases of double stranded DNA strands
How do you remove iodine stain from paper?
The iodine stain can be removed from the paper by washing the stain in plenty of cold water. Paper distillation can also be used to remove the iodine stain from the paper.
What is the importance of iodine after primary stain?
Iodine work as a modrant which helps in fixing the stain properly by making a compled compoun of stain and cell wall.
What will remove copper sulfate stain from porcelain?
what will remove copper sulfate stain in commodes
What does lugol's iodine stain do to the activity of the cell?
I just did a lab in biology called "How Plant and Animal Cells Differ" and in the packet it had that question. The answer is: Lugol's iodine stain stops the activity of the cell. It kills whatever specimen it is staining.
Role of ethidiumbromide in gel electrophoresis?
it is a visualising agent which enables you to watch the seperated DNA under the u.v light
How feulgen stain DNA and RNA?
fuelgen stain DNA
What dye is used to stain the bands of DNA?
it depends on the animal
What region of the DNA does the giemsa stain dye?
Giemsa stain binds the regions with high content of adenine-thymine complex.
What makes DNA visible?
You can an electrophoresis gel and then stain the gel using a solution such as coomassie blue to make the bands visible. Alternatively, you can stain a cell containing DNA by using acridine orange. It is necessary to observe these under an electron light microscope.
Will ethidium bromide stain double stranded DNA or single stranded DNA?
both, though DNA will preferentially form doublestranded DNA at room temperature. it can be quite hard to get single stranded DNA other than by heating your sample up to 95 degrees Celcius.
WHY DO YOU NEED TO STAIN THE DNA IN THE GEL?
When painting cabinets, it is important to stain the gel because it allows for the cabinet to protect itself against liquid oil bases. It provides an abrade to the surface.
What is nucleaer staining?
A stain selective for cell nuclei, usually based on the binding of a basic dye to DNA or to nucleohistone.
Use of acetocarmine in onion root tip squash?
Acetocarmine is a DNA specific stain like feulgen stain, so the super coiled chromosomes during different stages of mitosis present in the Onion root tip cells can be visualized perfectly by treating with this stain.
Why gel red can stain DNA fragments?
The DNA is a red color band under UV light on EtBr staining because EtBr intercalate and binds double stranded DNA unspecifically, when it absorbs UV light, it emits red color.
Why use acetocarmine stain?
we use acetocarmine to stain chtomosomes. stained chromosomes distinguish from the other organels and materials, it gives the chromosomes a red color. it enables that, during the cell cyle we can observe the nucleus, duplication of DNA, and movement.
What is the stain blue used to stain animal cells?
Methylene blue is used for many different staining purposes, but one of the main ones is staining RNA or DNA. In animal cells, it will stain the cytoplasm and the nucleus (the nucleus will be much darker).
