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What does ethidium bromide stain in a cell?
Ethidium bromide is a chemical that is able to intercalate (fit between the bases) between the bases of double stranded DNA strands
What is the importance of iodine after primary stain?
Iodine is used after the primary stain in the Gram stain procedure to form a complex with the crystal violet dye, which helps to stabilize the dye within the bacterial cell wall. This step enhances the retention of the primary stain in Gram-positive bacteria.
What is the purpose of ethidium bromide?
Ethidium bromide is an intercalating agent that attaches itself between the helix of a DNA. Because the ethidium molecule lights up when illuminated by an ultraviolet light, it is used often in biochemistry laboratories so that fragment of DNA that has been separated by gel can be visualized.
What does lugol's iodine stain do to the activity of the cell?
I just did a lab in biology called "How Plant and Animal Cells Differ" and in the packet it had that question. The answer is: Lugol's iodine stain stops the activity of the cell. It kills whatever specimen it is staining.
Why ethidium bromide is carcinogenic?
The purpose of ethidium bromide is as an intercalating agent commonly used as a fluorescent tag (nucleic acid stain) in molecular biology laboratories for techniques such as agarose gel electrophoresis. When exposed to ultraviolet light, it will fluoresce with an orange color, intensifying almost 20-fold after binding to DNA. Hence it is useful in visualizing DNA. Unfortunately, ethidium bromide does not distinguish between what DNA it binds to, whether it be ours or our sample's. Hence ethidium bromide is a mutagen, suspected carcinogen and at high concentrations is irritating to the eyes, skin, mucous membranes and upper respiratory tract. Ethidium bromide acts as a mutagen because it intercalates into double stranded DNA, thereby deforming the molecule. This is believed to block or trip biological processes occurring on DNA, like DNA replication and transcription.
How feulgen stain DNA and RNA?
The Feulgen stain is specific for DNA as it targets the deoxyribose residues in the DNA backbone. The stain reacts with the aldehyde residues in DNA to form a complex that fluoresces when exposed to UV light. RNA does not contain deoxyribose residues, so it does not bind to the Feulgen stain.
Role of ethidiumbromide in gel electrophoresis?
Ethidium bromide is commonly used in gel electrophoresis to visualize DNA under UV light. It intercalates between DNA base pairs, causing the DNA to fluoresce when exposed to UV light. This allows researchers to see and analyze DNA bands in the gel.
What dye is used to stain the bands of DNA?
it depends on the animal
What region of the DNA does the giemsa stain dye?
Giemsa stain binds the regions with high content of adenine-thymine complex.
How does DAPI stain work to visualize DNA in cells?
DAPI stain works by binding to the DNA in cells, causing it to emit a blue fluorescence when exposed to ultraviolet light. This allows researchers to visualize and study the DNA within the cells under a microscope.
How effective is the gel red stain in detecting DNA in laboratory experiments?
The gel red stain is highly effective in detecting DNA in laboratory experiments. It is commonly used due to its ability to bind specifically to DNA and produce a bright fluorescent signal under UV light, making it easy to visualize and analyze DNA samples in gel electrophoresis.
What makes DNA visible?
You can an electrophoresis gel and then stain the gel using a solution such as coomassie blue to make the bands visible. Alternatively, you can stain a cell containing DNA by using acridine orange. It is necessary to observe these under an electron light microscope.
Will ethidium bromide stain double stranded DNA or single stranded DNA?
both, though DNA will preferentially form doublestranded DNA at room temperature. it can be quite hard to get single stranded DNA other than by heating your sample up to 95 degrees Celcius.
WHY DO YOU NEED TO STAIN THE DNA IN THE GEL?
When painting cabinets, it is important to stain the gel because it allows for the cabinet to protect itself against liquid oil bases. It provides an abrade to the surface.
What is seminal stain?
A seminal stain is a biological fluid stain caused by semen. It is often analyzed in forensic investigations to determine the presence of semen on a surface or item. DNA analysis can be conducted on seminal stains to identify the donor.
What is nucleaer staining?
A stain selective for cell nuclei, usually based on the binding of a basic dye to DNA or to nucleohistone.
Use of acetocarmine in onion root tip squash?
Acetocarmine is a DNA specific stain like feulgen stain, so the super coiled chromosomes during different stages of mitosis present in the Onion root tip cells can be visualized perfectly by treating with this stain.
