First gentian violet or crystal violet solutn is applied,they bcome purple now iodine is added and acetone or ethyl alcohol is applied gram -ve bacteria lose their color while gram positive bacteria don't
Gram-negative bacilli are a diverse group of bacteria that have a complex cell wall structure making them stain pink/red on a Gram stain. They include common pathogens like Escherichia coli, Pseudomonas aeruginosa, and Klebsiella pneumoniae. Identification of the specific type of gram-negative bacilli is important for appropriate treatment.
many gram positive bacilli
You do not " get " them, but gram positive bacteria are gram positive because their cell wall is of sufficient thickness and properly constructed to hold the Gram stain. They have one thick wall of peptidoglycan that holds the stain against being washed away. Gram negative bacteria have two thinner walls which allow the stain to be washed away, basically.
The answer to whether HIV gram-stain positive or negative is that HIV gram-stain is negative. They retain the light red or pink color after the stain.
The acid-fast stain is positive in the sample.
Gram positive
The acid-fast stain result is positive for the sample.
Yogurt contains mostly lactic acid bacteria, which are Gram-positive bacteria. This means they will stain purple under a Gram stain.
Contamination
By which type of stain colors it.
If iodine is not applied, both the gram-positive and gram-negative stains will appear to be gram-negative. The iodine acts as a mordant that helps to fix the crystal violet stain in the gram-positive bacteria, making them appear purple. Without iodine, the crystal violet stain can be easily washed out of both gram-positive and gram-negative bacteria, resulting in a pink or red color.
A negative result for a spore stain indicates that the organism does not form endospores. Endospore formation is a survival mechanism for some bacteria to withstand harsh environments, so a negative result suggests that the organism may be more susceptible to environmental stressors.