First gentian violet or crystal violet solutn is applied,they bcome purple now iodine is added and acetone or ethyl alcohol is applied gram -ve bacteria lose their color while gram positive bacteria don't
many gram positive bacilli
You do not " get " them, but gram positive bacteria are gram positive because their cell wall is of sufficient thickness and properly constructed to hold the Gram stain. They have one thick wall of peptidoglycan that holds the stain against being washed away. Gram negative bacteria have two thinner walls which allow the stain to be washed away, basically.
The answer to whether HIV gram-stain positive or negative is that HIV gram-stain is negative. They retain the light red or pink color after the stain.
Gram positive
positive
A negative result for the spores stain indicate that the gram-negative organism is present. A positive result for a spore stain indicates that a gram positive organism is present.
Contamination
By which type of stain colors it.
Gram-positive bacteria which take up the stain turn purple, while Gram-negative bacteria which do not take up the stain turn red.
If iodine is not applied, both the gram-positive and gram-negative stains will appear to be gram-negative. The iodine acts as a mordant that helps to fix the crystal violet stain in the gram-positive bacteria, making them appear purple. Without iodine, the crystal violet stain can be easily washed out of both gram-positive and gram-negative bacteria, resulting in a pink or red color.
Bacteria are gram positive or gram negative. Serratia happens to be a gram negative bacteria. They appear pink on a gram stain. Gram positive bacteria stain to a purple color on a gram stain. We can classify and ID bacteria using their gram stain and shape. Some antibiotics only work on gram negative bacteria and some only work on gram positive bacteria. It helps a doctor know which antibiotic to use.
Gram positive bacterial stain purple. They do not take up the counter stain.