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What substrate does nuclease act upon?
The substrate for nuclease is nucleic acid, such as DNA or RNA.
How does high salt concentration influence denaturation kinetics of DNA?
High salt concentration slows the denaturation of DNA because it stabilizes the charges
Why is it important to use the same restriction enzyme for both cells in recombinant DNA?
Restriction enzymes are endonucleases that digest the DNA at a sequence specific site. Hind III for example cut between two As in the sequence AAGCTT in the both strand forming a sticky end. If you use this enzyme to cut in your vector DNA, you have to use the same enzyme in the insert DNA so as they can ligate by DNA ligation. This is the important use of same restriction enzyme in cloning.
DNA cutting enzymes used in the repair of DNA damage?
DNA endonucleases are used to cut the DNA. They are specific enzyme that recognize the particular site of the DNA and digest them. DNA polymerase and DNA ligase are also involved in repairing DNA damage.
How do biologists make use of restriction enzymes?
They cut strands of DNA at specific sites.
What substrate does nuclease act upon?
The substrate for nuclease is nucleic acid, such as DNA or RNA.
What does DNA polymerases use as their substrate?
The main substrate for for Taq polymerase is magnesium chloride
Describe the difference between a single digest and a double digest?
Restriction enzymes (endonucleases) are used for a variety of reasons in molecular genetics, including obtaining a "map" and cloning DNA. Single digests consitute DNA being treated with one restriction endonuclease, whereas double digests contain 2 enzymes. At times, it is difficult (or not possible) to perform double digests ... especially when the 2 enzymes have very different requirements for their activities (e.g. salt concentration, temperature optimums, ...). If a DNA restriction map is known for a particular enzyme, and if the DNA is treated with this enzyme, then one can ascertain whether the digest was complete or not. However, if a restrictioin map is just being compiled, and if the DNA is treated with 2 enzymes in a double digest, at times difficulties may arise in determining the map if either (or both) enzymes did not completely digest the DNA.
What happens if you add too much proteinase k to a lysis buffer when performing DNA extraction?
it depends what "too much" is. a concentration up to 1mg/mL or slightly higher can be still ok. proteinase K should digest only proteins, leaving your DNA intact.
How does high salt concentration influence denaturation kinetics of DNA?
High salt concentration slows the denaturation of DNA because it stabilizes the charges
WHAT IS The role of peg in ligation buffer?
In a ligation reaction, the speed of the reaction is determined by the concentration of the substrate. PEG in a ligation reaction is a complex hydrophobic molecule that takes up most of the space in the reactions crowding the aqueous DNA and ligase. The less space available for DNA and ligase makes the reaction much more efficient.
Where is the DNA concentration in prokaryotic cells?
in your house
DNA fragments from a gel are transferred to a nitrocellulose paper during the procedure called Southern blotting What is the purpose of transferring the DNA from a gel to a nitocellulose paper?
to permanently attach the DNA fragments to a substrate.
Why is it important to use the same restriction enzyme for both cells in recombinant DNA?
Restriction enzymes are endonucleases that digest the DNA at a sequence specific site. Hind III for example cut between two As in the sequence AAGCTT in the both strand forming a sticky end. If you use this enzyme to cut in your vector DNA, you have to use the same enzyme in the insert DNA so as they can ligate by DNA ligation. This is the important use of same restriction enzyme in cloning.
DNA cutting enzymes used in the repair of DNA damage?
DNA endonucleases are used to cut the DNA. They are specific enzyme that recognize the particular site of the DNA and digest them. DNA polymerase and DNA ligase are also involved in repairing DNA damage.
If your nanodrop is giving you a plasmid DNA concentration of 178.9 ng/µL what is the volume of sample, you need to take in order to have 10 ng DNA final concentration?
This will give you info
What is the action of nuclease in the body?
nuclease helps to digest the nucleic acids such as DNA & RNA in the body.
