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In a ligation reaction, the speed of the reaction is determined by the concentration of the substrate. PEG in a ligation reaction is a complex hydrophobic molecule that takes up most of the space in the reactions crowding the aqueous DNA and ligase. The less space available for DNA and ligase makes the reaction much more efficient.
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What is the role of urea in lysis buffer?
In lysis buffer urea denature the protein and increase the solubility of protein.
Substance that causes a solution to resist changes in pH?
It is called a buffer. Depending on the reaction and reactants it could be any kind of acid or base that has the right characteristics to play the role of a buffer.
What is role of potassum cloride in Tkm1 buffer for DNA isolation?
for cell lysis
What is the role of sorbitol in DNA isolation?
what is the function of sorbitol in producing plant protoplasts
Role of tris in TE in DNA elution?
Tris is used as a buffering agent in the elution buffer.
What is the role of buffer solutions in complexometric titration?
Buffer solutions resist the change in PH
What is role of buffer solution in complexometric titration?
buffer solutions resists change in PH
What is the role of urea in lysis buffer?
In lysis buffer urea denature the protein and increase the solubility of protein.
What the role of sds in buffer?
to disrupt cell membranes
Substance that causes a solution to resist changes in pH?
It is called a buffer. Depending on the reaction and reactants it could be any kind of acid or base that has the right characteristics to play the role of a buffer.
Role of Cl in TE buffer solution?
TE buffer is a often used as a buffer solution in molecular biology, mainly in procedures involving DNA or RNA. The purpose of TE buffer is to solubilize DNA or RNA, while protecting it from degradation.
What is sodium carbonate role in cell culture?
It is used to buffer the medium (there is some phosphate buffer in medium too). the carbonate/hydrocarbonat buffer in medium is in equilibrium with the CO2 in the incubator (usually set on 5%)
What is role of potassum cloride in Tkm1 buffer for DNA isolation?
for cell lysis
What is the role of glycerol in the lysis buffer?
To protect protein during thawing and freezing
What is the role of disodium phosphate dihydrate in insulin?
Disodium hydrogen phosphate dihydrate has the role of a buffer in some insulin receipts.
What is the role of Tris-HCl in phage preparation by PEG precipitation?
To Prevent Possible protease contamination
What is the role of NaCl in TBST buffer?
In this case sodium chloride form an isotonic solution.
