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How is protein electrophoresis using ads-page similar to DNA electrophoresis using agarose gel?
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What is the main difference between protein electrophoresis and nucleic acid electrophoresis?
There are many similarities and differences between protein and DNA electrophoresis.Similarities:PAGE protein and DNA electrophoresis both cause separation by size, creating bands that are viewed by the scientist or a machine. The smallest segments more the fastest due to less friction with the surface of their medium or equipment.The movement of charges through the medium is what causes the DNA or proteins to move. Electrons move from the negative to positive end of the gel or capillary tube.Differences:In PAGE protein electrophoresis, a polyacrylamide gel is used to prevent convection from altering the movement of the proteins. If the proteins are charged, and there is a worry that the charge will affect the mobility of the protein segments, 1% SDS can be added to get rid of the mass/charge issue. This way, only the mass of the segment determines how far it moves. In DNA capillary electrophoresis, the size of the capillary is so small that it does not have room for convection to occur (it is only 20-50 microns wide). Thus, there is no medium in the capillary but DNA itself.In protein electrophoresis, the proteins are often dyed so their movement can be viewed with the naked eye, or a machine. With DNA capillary electrophoresis, DNA strands are made through DNA replication with dNTPs that are fluorescently labeled for the different nucleotides. Each base is labeled a different color. A fine laser lights up the DNA strand in the capillary tube and reads what color fluoresces. This is how the nucleotide is identified.Protein PAGE electrophoresis is used to determine the purity of a protein sample. It can also be used to see how large the chains are that make up a multi-chain protein if a denaturing agent is added. DNA electrophoresis is used to get the order of nucleotides in a DNA sequence. It is done by chopping the DNA sequence into many smaller bits and sequencing them, then putting them back together by lining them up according to sequence overlaps. This is called the "shotgun" method. Protein electrophoresis can figure out the order of about 15-20 amino acids by a similar method, but DNA electrophoresis can get up to 1000 nucleotides (~300 amino acids). DNA electrophoresis is limited by the low probability that the DNA sequence would be cut into a segment greater than 1000 nucleotides.
If a gel electrophoresis comparison of a mother and daughter is taken would it be more similar than between a brother and sister?
most likely
What is electrophorosis?
Electrophoresis is an analytical method frequently used in molecular biology and medicine. It is applied for the separation and characterization of proteins, nucleic acids and subcellular-sized particles like viruses and small organelles. Its principle is that the charged particles of a sample migrate in an applied electrical field. If conducted in solution, samples are separated according to their surface net charge density. The most frequent applications, however, use gels (polyacrylamide, agarose) as a support medium. The presence of such a matrix adds a sieving effect so that particles can be characterized by both charge and size. Protein electrophoresis is often performed in the presence of a charged detergent like sodium dodecyl sulfate (SDS) which usually equalizes the surface charge and, therefore, allows for the determination of protein sizes on a single gel. Additives are not necessary for nucleic acids which have a similar surface charge irrespective of their size. Characterizing samples by exploiting both differences in charge and size can yield much more information. It requires that the same sample is analyzed not only in one, but several gels. This technique is further explained in the chapter on Ferguson plots. The procedure is more laborious, however the use of an automated electrophoresis apparatus can make this a fast, routine procedure (Gombocz, Cortez: Appl. Theor. Electrophoresis 1995, 4, 197-209).
How does gel electrophoresis is used to make a DNA fingerprint?
Gel electrophoresis separates DNA fragment on the basis of their size. In DNA fingerprinting or DNA typing given sample is cut up with restriction enzymes and run through electrophoresis and results are analyzed to check for DNA polymorphism between the given sample and a sample form suspect. In nutshell gel electrophoresis is boon for the people in forensics.
The best way to classify two different organisms that have similar appearance and are found in the same environment is to?
use gel-electrophoresis to compare DNA patterns
How are beans and eggs similar?
They both have a lot of protein.
What causes the bands on your DNA gels?
During gel electrophoresis, the DNA moves along the agarose gel to the positive side of the box, and after a certain amount of time, the smaller DNA fragments travel the farthest (because they have an easier time navigating the pores of the gel) and so on, leaving behind a series of bands comprised of similar-sized DNA fragments.
What are some potential designs for a gel electrophoresis chamber?
a box and a chamber and a lid and a comb A gel electrophoresis can be made two different ways---horizontal or vertical. I'm doing a project with it right now, and honestly, I prefer the horizontal. I don't know how the vertical works, but the horizontal is pretty much a box inside a box with the lid and comb. The bigger box, or your chamber, should be large enough to fit all the wiring on each ends of it, and the smaller box, or the box, in the middle. Your box should have two removable walls on the ends in which the wells are facing, and slits on one end for the comb to go into. You should put the comb in and fill the box up with the gel, and when it dries, you take the comb out and take off both walls. The charge can then run through the walls and to the other side of the chamber.
Is tequila a protein?
No, there is no protein in tequila. Alcohol is primarily carbohydrates. Actually, there is a protein found in fruit flies that is called "tequila", it is similar to a protein in humans that is called neurotrypsin and it is important in long term memory.
How are the different types of RNA similar?
They all function in protein synthesis.
What structure is most similar to the protein shell in a virus?
a cell wall
Why the LDH isoenzymes can be separated by electrophoresis?
In the name of ALLAh , In order to answer this qustation Why the LDH isoenzymes can be separated by electrophoresis? .... we are going to answer this questions……….. 1) WHAT is ELECTROPHORESIS ? 2) BIOCHEMICAL COMPOSATION of LDH ? First .. I will introduce information about ELECTROPHORESIS :- What is Electrophoresis ? Electophoresis is aprocess of separating substance according to their electric charge which they carrying by introducing this substances in electrical media whish carrying out -ve and +ve poles . Electrophoresis is an analytical method frequently used in molecular biology and medicine. It is applied for the separation and characterization of proteins, nucleic acids and subcellular-sized particles like viruses and small organelles. Its principle is that the charged particles of a sample migrate in an applied electrical field. If conducted in solution, samples are separated according to their surface net charge density. The most frequent applications, however, use gels (polyacrylamide, agarose) as a support medium. The presence of such a matrix adds a sieving effect so that particles can be characterized by both charge and size. Protein electrophoresis is often performed in the presence of a charged detergent like sodium dodecyl sulfate (SDS) which usually equalizes the surface charge and, therefore, allows for the determination of protein sizes on a single gel. Additives are not necessary for nucleic acids which have a similar surface charge irrespective of their size BIOCHEMISTERY of LDH :- Lactate dehydrogenase (LDH) catalyzes the reaction below. This reaction provides an important source of NAD+ for cells undergoing anaerobic glycolysis. Pyruvate + NADH + H+ Lactate + NAD+ LDH is a tetrameric protein consisting of two types of subunits, called M and H, which have small differences in amino acid sequence. Different molecular forms of an enzyme are called isoenzymes or isozymes. M subunits predominate in skeletal muscle and liver, and H subunits predominate in heart. M and H subunits combine randomly with each other, so that the five major isoenzymes have the compositions M4, M3H, M2H2, MH3, and H4. Because of random subunit reassortment, the isoenzymic composition of a tissue is determined primarily by the activities of the genes specifying the two subunits. This propartey can indicate why LDH has electrophorises activity. ABD ELRHMAN OSAMA , abdelrhmanosama@yahoo.com , 4th year faculty of medicine , Al-azher univ., EGYPT .
