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What are some potential designs for a gel electrophoresis chamber?
Wiki User
∙ 11y ago
a box and a chamber and a lid and a comb
A gel electrophoresis can be made two different ways---horizontal or vertical. I'm doing a project with it right now, and honestly, I prefer the horizontal. I don't know how the vertical works, but the horizontal is pretty much a box inside a box with the lid and comb. The bigger box, or your chamber, should be large enough to fit all the wiring on each ends of it, and the smaller box, or the box, in the middle. Your box should have two removable walls on the ends in which the wells are facing, and slits on one end for the comb to go into. You should put the comb in and fill the box up with the gel, and when it dries, you take the comb out and take off both walls. The charge can then run through the walls and to the other side of the chamber.
Wiki User
∙ 11y ago
Wiki User
∙ 14y agoDuring DNA electrophoresis DNA and restriction enzymes are inserted into the wells of a agarose gel. The agarose gel is then placed into a electrophoresis chamber along with a buffer (the buffer keeps the DNA fragments soluble in water). The electrophoresis chamber has a electrical charge running through it that carries the DNA fragments through the gel. Because the gel is porous DNA fragments can travel through it, but the varying sizes of the fragments means that some can travel farther than other--as they are all cut by the restriction enzymes at certain locations. The result is a DNA stain, similar to what you would see in TV or movies. In short the electrophoresis chamber houses the gel as the current is run through it so that a DNA stain can be obtained.
Wiki User
∙ 12y agoIt is a box which contains a gel which is used to separate various sizes of DNA, RNA, or proteins from each other. The gel is actually a polymer matrix of various components depending on what you want to separate (DNA, RNA, etc.) An electric current is applied to this matrix which causes the strain to separate.
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What is the purpose of gel electrophoresis?
The buffer is the medium through which the current flows. In the electrophoresis chamber, the anode and cathode are separated and the gel is placed between them. In order to close the circuit and generate the voltage which causes the migration, the entire chamber is filled with a conductive buffer. It is actually possible to perform electrophoresis without a buffer; however this requires a specially made electrophoresis chamber. In these chambers the electrodes actually contact the top and bottom of the gel eliminating the need for a conductive buffer to close the circuit.SDS PAGE electrophoresis uses buffer not primarily as a conductor but for holding a desired pH, dissipating heat and providing SDS in excess in the case of denaturing gels. A gel would run without a buffer as the gel itself is a conductor but the currents involved would heat it to the point of decomposition. Also the volume of liquid in a gel does not allow for an adequate pH buffering system. Holding a pH is extremely important for reproducibility especially in native gels as the pH can change the charge on the peptide. It is true some gels do not require buffer but these are rare cases like isoelectric focusing.the primary application of the buffer would be to conduct electricity,to form a closed circuit
What is the function of agarose gel electrophoresis?
Agarose gel electrophoresis is for determining the size of a piece of RNA or DNA. It can be used to determine the culprit of a crime, relatives, even the cause of some diseases, like Mad Cow.
Do anodes have a positive electricity charge?
The "anode" is usually considered to be "negative". However in some experiments such as Gel Electrophoresis the anode is positive.
What is the main difference between protein electrophoresis and nucleic acid electrophoresis?
There are many similarities and differences between protein and DNA electrophoresis.Similarities:PAGE protein and DNA electrophoresis both cause separation by size, creating bands that are viewed by the scientist or a machine. The smallest segments more the fastest due to less friction with the surface of their medium or equipment.The movement of charges through the medium is what causes the DNA or proteins to move. Electrons move from the negative to positive end of the gel or capillary tube.Differences:In PAGE protein electrophoresis, a polyacrylamide gel is used to prevent convection from altering the movement of the proteins. If the proteins are charged, and there is a worry that the charge will affect the mobility of the protein segments, 1% SDS can be added to get rid of the mass/charge issue. This way, only the mass of the segment determines how far it moves. In DNA capillary electrophoresis, the size of the capillary is so small that it does not have room for convection to occur (it is only 20-50 microns wide). Thus, there is no medium in the capillary but DNA itself.In protein electrophoresis, the proteins are often dyed so their movement can be viewed with the naked eye, or a machine. With DNA capillary electrophoresis, DNA strands are made through DNA replication with dNTPs that are fluorescently labeled for the different nucleotides. Each base is labeled a different color. A fine laser lights up the DNA strand in the capillary tube and reads what color fluoresces. This is how the nucleotide is identified.Protein PAGE electrophoresis is used to determine the purity of a protein sample. It can also be used to see how large the chains are that make up a multi-chain protein if a denaturing agent is added. DNA electrophoresis is used to get the order of nucleotides in a DNA sequence. It is done by chopping the DNA sequence into many smaller bits and sequencing them, then putting them back together by lining them up according to sequence overlaps. This is called the "shotgun" method. Protein electrophoresis can figure out the order of about 15-20 amino acids by a similar method, but DNA electrophoresis can get up to 1000 nucleotides (~300 amino acids). DNA electrophoresis is limited by the low probability that the DNA sequence would be cut into a segment greater than 1000 nucleotides.
How do you know which molecule is the smallest in a DNA fingerprint?
The smallest molecule travels fastest once the current is switched on.If the process is slab gel electrophoresis, the smallest molecule (or rather, about 200 million of them following PCR or some other process of amplification) will be the one nearest the positively-charged end of the gel.If the analysis is by capillary electrophoresis, the smallest molecule will be the first one to pass the window and interrupt the laser beam.
What are the common stains that is used after DNA electrophoresis?
Ethidium bromide and coomassie blue are some stains that can be used in DNA electrophoresis.
What is the aim of electrophoresis?
It is used to separate molecules by some properties
Can you put a turbo on a carbureted motor?
yes, it can be done with extensive modification of carburetor or by replacing it with specially designed carburetors. some designs put carburetor inside pressurized plenum chamber.
Some viva voce questions for electrophoresis?
1. WHAT IS ELECTROPHORESIS AND WHAT ARE THE IMPORTANTAPPLICATIONS OF ELECTROPHORESIS?Ans. Movement of charged particle in the electric field either towards cathode or anode whensubjected to an electric current is called electrophoresis.The following factors influence the movement of particles during the electrophoresis.(a) Electric current.(b) Net charge of the particle.(c) Size and shape of the particle.(d) Type of supporting media.(e) Buffer solution.Important Applications of ElectrophoresisThe technique of electrophoresis is used to separate and identify the(i) Serum proteins(ii) Serum lipoproteins(iii) Blood hemoglobins2. WHAT ARE THE DIFFERENT TYPES OF ELECTROPHORESIS?Ans. (a) Moving boundary electrophoresis: This technique was first introduced by TISELIUS in 1937(b) Zone electrophoresis: In this type of electrophoresis different types of supporting mediaare used. These are;(a) Paper electrophoresis(i) Whatman filter paper(ii) Cellulose acetate(b) Gel electrophoresis(i) Agarose.(ii) Polyacrylamide gel (used for the separation of isoenzymes).(iii) SDS-PAGE.(iv) Iso-electric focussing (proteins seperated in a medium possessing a stable pH gradient).(v) Immuno electrophoresis (for the separation of immunoglobulins).
What are some separation variables with the molecules in electrophoresis?
hi. i dont know the question. ok bye.
For what purpose might one use capillary electrophoresis?
Capillary electrophoresis is a technique used in laboratories to separate molecules based on their charge in order to study and analyze them. Capillary electrophoresis uses an electric charge to force the movement of molecules since each molecule will go a varying distance based on the weight of the molecule and their charge. Some areas of study that use capillary electrophoresis include DNA sequencing and pharmaceutical analysis.
Where can I get some ideas for new bathroom designs?
My bathroom is looking terrible. Where can I get some ideas for some new bathroom designs?
Is chamber an adjective?
No. Chamber is a noun. In some uses, chambered can be the adjective.
Does gel electrophoresis affect the case of either suspect?
This question is not complete, it lacks some information for me to correctly answer it.
What are some applications for gel electrophoresis used?
Paternity testing and crime lab applications (DNA matching) etc
What are some designs?
It depends, your question includes a wide variety, Designs are like setups, shapes, etc. There are many designs for many things.
Where can designs be made for a house?
Designs for a house can be made with architectural software. You can do this yourself at home or hire an architect to draw up designs for you. Some premade designs are available on the internet as well.
