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How many samples of DNA were placed in each well in the agarose gel electrophoresis?
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Process that restriction fragments of DNA are separated from each other by the use of electricity?
The process is referred to as gel electrophoresis. This is an analytical process where DNA fragments can be separated based on size within a gel under the influence of an electric field
How does agarose gel electrophoresis work?
Gel electrophoresis is the process by which molecules in a sample can be separated by charge and/or size. Firstly, agarose gel is prepared in a casting tray by placing the comb in the middle of the gel and placing end blocks on either end of the tray. After this solution has settled, the end blocks can be removed along with the comb. After the comb is removed, wells should be present within the agarose gel. Next, a buffer solution should be placed into the electrophoresis chamber; this solution conducts electricity which is needed in order to separate the molecules from the samples. Then (using a micropipette) each of the samples in the experiment will be loaded into a corresponding well in the agarose gel. Afterwards, the leads on the electrophoresis chamber must be connected to a power source; the process of gel electrophoresis will then begin.
Why would other bands be present in gel electrophoresis if they are not supposed to be present?
This answer assumes that prior to the electrophoresis, you have applied a restriction enzyme to the DNA which breaks it up into fragments of different lengths. Electrophoresis separates fragments of DNA according to their molecular mass, size and charge. Each band will represent a pool of fragments that are the same length. The shortest, lightest fragments will travel the furthest through the gel, where as the long, heavy fragments will not travel very far. The darkness of the band also indicates the frequency of that particular length fragment.
What do DNA bands represent in the agarose gel electrophoresis?
Each band represents a piece of DNA. The extent to which they move through the gel has to do with the fragment's electrophoretic mobility. The lighter the molecule in general the faster it can move through the gel. Usually when performing a gel electrophoresis one would use markers. These markers would be of known molecular weight and would allow you to compare your DNA fragments and find approximate molecular weights.
What are the similarities between paper chromatography and electrophoresis?
Chromatography is a collective term for a family of lab techniques for the separation of the mixtures.It involves a passing a mixture dissolved in a mobile phase through a stationary phase. Electrophoresis is the process by which molecules (such as proteins, DNA, or RNA fragments) can be separated according to size and electrical charge by applying an electric current to them. Each kind of molecule travels through the medium at a different rate, depending on its electrical charge and molecular size In the electrophoresis techniques electricity is required and positive charge goes to the cathode whereas the negative charges goes to the anode (opposite charges attraction) but in Chromatography there is no need for the current or electricity .
Which statement best describes the relationship between a heat sink and a heat source if the samples are placed in contact with each other?
4. The dry air will be a heat source for the water
How are restriction enzymes used to look for differences between DNA samples?
Restriction enzymes cleave, or open, the DNA so that a sample can be taken and gel electrophoresis can separate the strands of DNA. From there, DNA probes bind to certain strands in each sample and DNA fingerprints can show the differences.
What if two samples of elements each represent one mole then?
If two samples of elements each represent one mole, then both samples have 6.022 x 1023 atoms of the elements.
How many fossil kits with 12 samples each have the same number of fossils as 30 fossil kits with 8 samples each?
20
What are some potential designs for a gel electrophoresis chamber?
a box and a chamber and a lid and a comb A gel electrophoresis can be made two different ways---horizontal or vertical. I'm doing a project with it right now, and honestly, I prefer the horizontal. I don't know how the vertical works, but the horizontal is pretty much a box inside a box with the lid and comb. The bigger box, or your chamber, should be large enough to fit all the wiring on each ends of it, and the smaller box, or the box, in the middle. Your box should have two removable walls on the ends in which the wells are facing, and slits on one end for the comb to go into. You should put the comb in and fill the box up with the gel, and when it dries, you take the comb out and take off both walls. The charge can then run through the walls and to the other side of the chamber.
For what purpose might one use capillary electrophoresis?
Capillary electrophoresis is a technique used in laboratories to separate molecules based on their charge in order to study and analyze them. Capillary electrophoresis uses an electric charge to force the movement of molecules since each molecule will go a varying distance based on the weight of the molecule and their charge. Some areas of study that use capillary electrophoresis include DNA sequencing and pharmaceutical analysis.
What does it mean if two DNA sample showed an identical pattern and thickness of been produced by Gel electrophoresis?
By the same amount of DNA, fragments of the same size and the same DNA molecules. WRONG The real answer on the people who have multiple choice or e2020 It would be "all the above"
