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we started with a single strand DNA molecule.
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Difference between DNA replication and transcription?
1. Replication is the duplication of two-strands of DNA. Transcription is the formation of single, identical RNA from the two-stranded DNA. 2. There are different proteins involved in replication and transcription. 3. In replication, the end result is two daughter cells, while in transcription, the end result is a protein molecule. 4. In transcription, DNA serves as the template for RNA synthesis.
How is DNA replicated-?
In a cell, DNA replication must happen before cell division can occur. DNA synthesis begins at specific locations in the genome, called "origins", where the two strands of DNA are separated. RNA primers attach to single stranded DNA and DNA polymerase extends from the primers to form new strands of DNA, adding nucleotides matched to the template strand. The unwinding of DNA and synthesis of new strands forms a replication fork. In addition to DNA polymerase, a number of enzymes are associated with the fork and assist in the initiation and continuation of DNA synthesis by joieman04
How the end results of transcription and replication differ?
In the replication part, replication ensures that each new cell will have one complete set of genetic instructions. it does this by making identical strands of chromosomes. transcription enables to adjust to changing demands. it changes certain types of strands on the DNA
What do DNA polymarase do in DNA?
DNA polymerase is responsible for the process of DNA replication, during which a double-stranded DNA molecule is copied into two identical DNA molecules. Scientists have taken advantage of the power of DNA polymerase molecules to copy DNA molecules in test tubes via a polymerase chain reaction, also known as PCR. Creative Biogene
How are DNA and RNA replicated?
During interphase.
At the end of DNA replication two new strands of DNA have been produced giving a total of?
Two new DNA chains are formed at the end of DNA replication, both identical in sequence to the template (or parent) chain. These chains are composed of 2 complimentary strands. It is important to note that of the newly formed DNA chains, one strand is the same as the template strand and the other one is a newly synthesized one.
What end product is formed during replication?
two identical strands of DNA
DNA molecule antiparallel Why?
The DNA molecule is anti-parallel. This is because the two strands are the opposite of one another, such that if one strand has the base sequence ATC, the opposite strand would have the base sequence TAG.
One function of gel electrophoresis is to?
To separate strands of DNA based on their size. Shorter strands will migrate more slowly than larger strands. ** Also because DNA is slightly negatively charged, it will move toward the positive end of the electrodes... this is why the current is used when running a gel. Short strand move further** than large ones due to the gel resistance.
The direction of DNA replication on the leading strand occurs in the?
The enzyme DNA polymerase synthesises strands in the 5 prime to 3 prime direction, and as DNA is antiparallel the replication of the leading strand occurs from the 3 prime end of the template to the 5 prime end of the template.
Where do the 2 strands of the parent DNA end up in the daughter chromosome after DNA replication?
To simplify: the DNA molecule unzips and divides into 2 strands, then the replication makes the complements of each strand to make 2 full DNA daughter chromosomes, so half the parent DNA ends up in each daughter chromosome.
What happens first during transcription?
RNA polymerase reaches the beginning of the gene
Difference between DNA replication and transcription?
1. Replication is the duplication of two-strands of DNA. Transcription is the formation of single, identical RNA from the two-stranded DNA. 2. There are different proteins involved in replication and transcription. 3. In replication, the end result is two daughter cells, while in transcription, the end result is a protein molecule. 4. In transcription, DNA serves as the template for RNA synthesis.
What okazaki fragments are?
In DNA replication, the two DNA strands acting as templates need to be synthesized simultaneously. DNA polymerase is an enzyme which can synthesize the DNA only in 5' to 3' direction.the two template strands are anti-parallel to each other and their complementary strands are synthesized in different direction. In one of the strand DNA is synthesized continuously by adding nucleotides at 3'-OH end. this is referred as 'leading strand' synthesis. the other strand to be synthesized is replicated in short fragments referred 'Okazaki fragments' named after their discoverer Reiji Okazaki.
How does RNA differantiate from DNA if it is double strnded?
the following points differentiates double stranded RNA And DNA : 1. the RNA is loop structure at the end and not that independent strands of the DNA. this loop is calles R-loop, t includes a sequence if repeated poly A tail. 2. it contains poly a tail at the end but DNA doesn't.
What are two ways that the end result of transcription and replication of DNA differ?
In the replication part, replication ensures that each new cell will have one complete set of genetic instructions. it does this by making identical strands of chromosomes. transcription enables to adjust to changing demands. it changes certain types of strands on the DNA
What are the step of replication?
The steps of DNA replication: 1. Unwinding Each DNA molecule consists of two strands. These strands seperate from each other. 2. Complementary pairing up of the nucleotide bases. 3. Enzymes link up and join. 4. Termination This is when DNA polymerase to an end of the strands.
