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Q: How the vector pbr322 contain resistance?
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What is the cloning capacity of pBR322 vector?

The cloning capacity of pBR322 vector is 1-5kb.


Explain the functioning of pBR322 vector with diagram?

FUNCTIONG OF pBR322


What is the advantages and disadvantages of pBR322 as a cloning vector?

pBR322 advantages is it widely used for the analysis of prokaryotic transcription and translation as well as topological changes in DNA conformation. then the disadvantage is it has only few cloning sites and the selection procedure is therefore time consuming.


Is resistance a vector?

A vector quantity is one that has a magnitude (a number), and a direction. No, resistance is not a vector quantity; it is a scalar quantity (only magnitude).


Does a cloning vector contain promoter region?

No, not really since it is just for cloning. But their should be enough promoter/sequence to provide antibiotic resistance.


WHAT IS pBR322?

pBR322 is one of the most used cloning vectors in molecular biology. Cloning vectors, best-known as plasmids, are autonomously replicating DNA units into which DNA fragments can be inserted for gene cloning. Genes taken up by these plasmids are multiplied (or cloned) as the vector replicates, to yields numbers suitable for molecular analysis. The most versatile and well-known plasmid is certainly pBR322 (in fact was one of the first ever used in gene cloning techniques) and has genetically tailored cutting sites into which DNA can be inserted without affecting plasmid self-replication. pBR322 general characteristics are: a) Size: 4.3 kb; b) Replicon: ColE1, relaxed; c) Selective markers (resistance): Amp and Tet; d) Single sites (enzymatic restriction single sites): Ava I, Pst I, BamHI, PvuII, ClaI, SalI, EcoRI, and HindIII.


How can you know donored-vector is cloning vector or expression vector?

Expression vectors contain translation start and termination codons. Moreover, it contains promoter region


What two components does a vector contain?

A magnitude (size) and a direction.


Can alternating current be expressed as a vector?

In the case of AC, you can express the current, the voltage and the equivalent to the resistance (called "impedance" in this case) as a vector - with a magnitude, and a phase angle.


How many bands were produced after pBR322 was digested with EcoR1?

pBR322 has one EcroR1 site so 1 band however if it was not fully digested you will find 2 or 3 (Linear- [cut], Supercoiled-, Round-Plasmid).


What is meant by the term capacitive resistance?

That term to me is incorrect it should be capacitance impedance. Resistance is linear impedance. CAPACITANCE will follow a vector caused by the capacitor value.


Can the triangle law of vector addition be used for many vectors?

no because triangle only contain three vectors and if many vector are added then they cant form a triangle