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when you plate E.coli onto a EMB plate the colonies turn shinie metalic green, EMB is selective for gut bacteria and it diferentiates E.coli.

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15y ago

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Can Kliglers Iron Agar tell the difference between e coli and e aerogenes?

Yes, Kligler's Iron Agar (KIA) can help differentiate between Escherichia coli and Enterobacter aerogenes. E. coli typically ferments glucose and lactose, producing acid throughout the medium, resulting in a yellow slant and butt. In contrast, E. aerogenes ferments glucose and lactose as well, but often produces gas, which can lead to cracks or lifting in the agar, and may also show a yellow slant and butt, but with different gas production characteristics. Thus, KIA can provide valuable information for distinguishing between these two organisms.


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