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Q: How would mutation affect restriction enzymes?
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Which pair of enzymes is necessary to make recombinant DNA?

Actually the answer would be Restriction enzyme and DNA ligase.


How can a mutation that alters a recognition site be detected in gel electrophoresis?

The point mutation has to result in either the removal of a restriction site of the restriction enzymes or the formation of a new one, such that the bands of mutated DNA that form after performing gel electrophoresis are different from the normal one. So a difference in banding patterns would mean that there is a point mutation.


How would a mutation affect the next generation?

If the mutation were in the gametes. Male and female reproductive cells.


What would most unpredictably affect an ecosystem?

a mutation in native bacteria


How does a mutation in a cell affect an organism?

A mutation in a sex cell means that the mutation can be passed on to the individuals offspring. If the mutation just occurred in a somatic cell, it would not be passed down.


A mutation in the genes for salivary amylase will greatly affect the chemical digestion of what?

Amylase is responsible for the digestion of starches in the body. If a mutation occurred in the genes coding for the production of amylase, this would interfere with the body's ability to digest starches and other complex carbohydrates (which begins in the mouth with enzymes from the salivary glands).


Why do bacteria naturally contain restriction enzymes?

Restriction enzymes are the bacteria's form of an 'immune system' against viruses (which can infect bacteria). When viruses try to insert their own DNA into a bacteria's genome, the restriction enzymes detect this foreign DNA and cut it out so that the viruses can't replicate and kill the cell.


What substance is used to cut DNA?

restriction endonuclease


Assuming a circular piece of DNA was used as starting material how many restriction sites were there in lane three?

It takes one restriction enzyme to cut a linear piece of DNA (straight line) into two pieces. For a circular piece of DNA (plasmid), it would take two of these restriction enzymes - just think of how you would split a circle into two pieces; cutting one section will only straighten out the DNA, not split it (try cutting a rubber band for a visual).The answer to this question is completely dependent on the number of bands (representing different DNA fragment sizes) the lane produced. Since the first cut on a circular piece of DNA breaks the circle, then each consecutive cut will proceed as a linear band of DNA would.For example, if lane three produced four bands, then it took a total of three restriction enzymes (each enzyme reacting to one restriction site). Below is an example of the linear piece of DNA from lane three, with each "|" representing a restriction site. Notice how there are three restriction sites, but four fragments ("----") are produced.---- | ---- | ---- | ----As explained earlier, if a circular piece of DNA were cut to produce a linear piece like the one above, it would take one restriction site. From there, the enzymes proceed like the ones in the above example. Therefore, a circular piece of DNA that produced four bands would have used four restriction enzymes, whereas a linear piece of DNA that produced four bands would have used three restriction enzymes.


What mutations would most likely affect a person's offspring?

Any mutation could do so.


Why does an insert mutation usually cause more defects during protein synthesis than a point mutation?

Insertion mutations can affect many amino acids in the protein.An insertion mutation usually causes more defects during protein synthesis than point mutation because an insertion mutation will affect many amino acids in the protein.


What would the consequences of a mutation that prevented a person's body from making a membrane protein necessary for active transport of sugar from the digestive system?

A disease known as hypoglycemy would affect the human who gets the mutation. The body would not be able to obtain energy from carbohydrates and would, therefore, be greatly deprived of energy. A disease known as hypoglycemy would affect the human who gets the mutation. The body would not be able to obtain energy from carbohydrates and would, therefore, be greatly deprived of energy.