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restriction endonuclease

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Chauncey Kihn

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1y ago
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12y ago

Scientists usually use chemicals that contain restriction enzymes. Restriction enzymes target certain sequences of DNA where they break the bonds of a certain area of the sequence. For example if a scientist were to use a restriction enzyme that cuts DNA wherever the sequence AATT is into AA TT. On a certain peice of DNA that looks like this ATGGCAATTGCCGTA the DNA would then break like this ATGGCAA TTGCCGTA

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Q: What substance is used to cut DNA?
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What is the name of the substance that is used to cut DNA at particular sequence?

DNA is cut by a special kind of enzymes called restriction enzymes.


What is the name of the substance that is used to cut DNA at particular sequences?

DNA is cut by a special kind of enzymes called restriction enzymes.


Why you do electrophoresis?

electrophoresis is the process of putting dyed DNA that has been cut by enzymes into a gel substance in order to seperate the DNA for genetic ID. It can be used for paternity testing comparing DNA of the child to the father.


What type of enzyme is used to fragment dna?

restriction enzymes are used to cut DNA.


what substance was needed to join the insulin gene to the bacterial DNA?

Technically it is not a substance, but the DNA itself. Let me explain. When the insulin gene is cut out of a regular strand, it is done through a DNA ligase (a cutting enzyme). The SAME ligase is used to cut the bacterial loop of DNA. When ligase cuts DNA sticky ends are left. These sticky ends are, as they say, sticky, and will readily join to new bases with the corresponding (complementary) base pair sequence. As the same ligase is used, the corresponding base sequence is inside the bacterial DNA, so they should connect together.


What part of speech is the word DNA?

The word DNA is a noun. It is a substance used to identify a person.


What do scientists used to cut DNA?

scissors or their teeth


How is DNA manipulated?

Chemical mutagens are used to change DNA sequences, (bacterial) enzymes are used to cut and copy DNA, and repressors are used to prevent the expression of genes.


Explain how a DNA fingerprinting produced?

In DNA fingerprinting, enzymes are used to cut the DNA in the smaple found at the crime scence


What part of speech is DNA?

The word "DNA" is a noun. It is a substance unique to each living person that can be used to identify them.


What chemicals are used to cut DNA into fragments?

The chemicals used to cut DNA fragments are restriction endonucleases, eg- Eco R I, Eco R II,etc


Enzymes used to cut DNA molecules in recombinant DNA researsh are?

restriction enzymes