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Well if you stained with only crystal violet, then they would all be violet! If you do a Gram Stain the right way, you end up getting Gram positive being violet or purple and Gram Negative being red or pink.
Wiki User
∙ 15y ago
Wiki User
∙ 13y agoimportant in distinguishing between gram positive and gram negative bacteria.
Process:
1. Dye the bacteria with crystal violet
2. add iodine
3. wash with alcohol to decolourise
4. the gram positive should stay violet. the gram negative will become colourless.
much love
The SS x
Wiki User
∙ 13y agoCrystal violet stains G+ bacteria and G- bacteria at the beginning of the gram staining process. It is later washed away from the G- bacteria by the alcohol/acetone rinse.
Shorter Answer:
Stains G+ bacteria
Wiki User
∙ 11y agocrystal violet is consider a simple stain so it gives the same color to all bacteria, a gram stains is what divides the bacteria into gram + or gram negatives
Wiki User
∙ 15y agowhen we put crystal violet on the smear containing microorganisms,it stains all the gram positive as well as the gram negative organisms violet.
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What is the purpose of crystal violet in the Gram's stain procedure?
Its the primary stain of the procedure. IT stains the Gram positive organisms
What is the function of iodine solution in the gram stain?
It allows the primary stain crystal violet to remain in the cell instead of being washed out. Due to the larger size of the crystal violet molecule, when the ethanol is applied (the decolorizer) the stain will not be washed out of the Gram (+) positive cells.
Function of acetone in gram staining?
Acetone is used for decolouring method, washing away the iodine-crystal violet complex formed in gram negative bacteria. so that the gram negative bacteria can be then stained with safranin or fuchsin.
What is a decolorizer do in gram staining?
It removes the crystal violet (primary stain) from gram negative bacteria. It does not remove Crystal violet as easily from gram positive bacteria, because the highly peptidoglycan walls of gram positive bacteria interact with crystal violet and iodine to form a strong bond (CV-Iodine complex). So, gram positive cells hold on to the stain instead of letting it wash away.
Purpose of decolorizing agent?
The decolorizer, usually acetone or alcohol, is used to wash the Crystal Violet stain from the Gram Negative cells. From this point Safranin stain is used to stain the Gram Negative cells. The final color for Gram Negative will be a Red/Pink color.
What is the secondary stain in gram stain procedure?
It is crystal violet & stains all cells purple.
What color will gram stain positive turn into?
If using a gram stain, they will turn Crystal Violet.
What function of iodine in gram stain?
fixing the stain so that the first dye which is the crystal violet will not be washed away during rinse process.
What is the purpose of the crystal violet in the gram stain procedure?
Its the primary stain of the procedure. IT stains the Gram positive organisms
What is the purpose of crystal violet in the Gram's stain procedure?
Its the primary stain of the procedure. IT stains the Gram positive organisms
What is the function of iodine solution in the gram stain?
It allows the primary stain crystal violet to remain in the cell instead of being washed out. Due to the larger size of the crystal violet molecule, when the ethanol is applied (the decolorizer) the stain will not be washed out of the Gram (+) positive cells.
What is crystal violet iodine complex?
In Gram staining procedure during bacterial staining , iodine forms a complex with crystal violet stain which stains Gram positive bacteria blue to violet .
Function of iodine in Gram stain?
iodine act as a mordant..on the gram positive bacteria which got really thick and abundence of peptidoglycan layer, the crystal violet will fix to the peptidoglycan layer..meanwhile in gram negative bacteria which is lack of peptidoglycan layer, the alcohol or acetone will wash it away
What function of lugol's iodine in gram staining?
fixing the stain so that the first dye which is the crystal violet will not be washed away during rinse process.
Function of acetone in gram staining?
Acetone is used for decolouring method, washing away the iodine-crystal violet complex formed in gram negative bacteria. so that the gram negative bacteria can be then stained with safranin or fuchsin.
Why does the counterstain not change the color of all of the cells is it because the primary stain repels or masks the secondary stain?
In a gram stain the primary stain is crystal violet. Iodine then sets that dye into the gram positive cells while alcohol washes out the crystal violet from the gram negative cells. Then safranin, which is the counterstain in a gram stain, is used to dye the rest of the bacteria. This is the example I can give you of why a counterstain does not change the look in all the cells. Though safranin stains all the cells, the gram positive cells that were dyed purple from crystal violet don't look pink - only the gram negative do.
Function of crystal violet?
Crystal violet is a hexamethyl also known as methyl violet 10B This is much darker than 2B, and often darker than 6B. It is used in biological stainang particularly gram staining together with safranin and iodine. Crystal violet was also used to treat strept throat. Doctors used to swab it on the back of your throat.
