0
iodine act as a mordant..on the gram positive bacteria which got really thick and abundence of peptidoglycan layer, the crystal violet will fix to the peptidoglycan layer..meanwhile in gram negative bacteria which is lack of peptidoglycan layer, the alcohol or acetone will wash it away
Wiki User
∙ 13y ago
Add your answer:
Earn +20 pts
What is crystal violet iodine complex?
In Gram staining procedure during bacterial staining , iodine forms a complex with crystal violet stain which stains Gram positive bacteria blue to violet .
How do you remove iodine stain from paper?
The iodine stain can be removed from the paper by washing the stain in plenty of cold water. Paper distillation can also be used to remove the iodine stain from the paper.
What is the importance of iodine after primary stain?
Iodine work as a modrant which helps in fixing the stain properly by making a compled compoun of stain and cell wall.
What does lugol's iodine stain do to the activity of the cell?
I just did a lab in biology called "How Plant and Animal Cells Differ" and in the packet it had that question. The answer is: Lugol's iodine stain stops the activity of the cell. It kills whatever specimen it is staining.
Diff between gram's iodine and iodine?
iodine& grams iodine Gram's iodine is a solution of 2% iodine and 3% potassium iodide in 70% ethanol. Gram's iodine is also used as a forensic toxicology reagent for the detection of alkaloids, as a source of iodine for iodometric titrations, as an indicator for the presence of starch, and as a general laboratory source of iodine/iodide. This solution is laboratory grade, and not for medicinal use. Iodine is non metallic,dark gray,lustrous,solid element
Why do you use iodine to stain a cell?
Iodine stains starch contained in cells. Iodine is also used to distinguish between Gram negative and Gram positive bacteria. The Gram stain contains iodine.
What is the use of Iodine in gram stains?
The iodine in the gram stain serves as a mordant or fixative.
What function of iodine in gram stain?
fixing the stain so that the first dye which is the crystal violet will not be washed away during rinse process.
What color is gram negative stain and gram positive stain if you forget to apply iodine?
If iodine is not applied, both the gram-positive and gram-negative stains will appear to be gram-negative. The iodine acts as a mordant that helps to fix the crystal violet stain in the gram-positive bacteria, making them appear purple. Without iodine, the crystal violet stain can be easily washed out of both gram-positive and gram-negative bacteria, resulting in a pink or red color.
What is the function of iodine solution in the gram stain?
It allows the primary stain crystal violet to remain in the cell instead of being washed out. Due to the larger size of the crystal violet molecule, when the ethanol is applied (the decolorizer) the stain will not be washed out of the Gram (+) positive cells.
What function of lugol's iodine in gram staining?
fixing the stain so that the first dye which is the crystal violet will not be washed away during rinse process.
What is the action of iodine in grams stain?
Iodine is used to bind the Crystal Violet to the Gram Positive microbes.
Why gram's iodine called a mordant?
Gram's iodine is called a mordant because it fixes the primary stain to the cell wall and adds any color.
Why is gram's iodine called mordant?
Gram's iodine is called a mordant because it fixes the primary stain to the cell wall and adds any color.
What is the secondary stain in gram stain procedure?
It is crystal violet & stains all cells purple.
What is the difference between gram's stain and wright's stain?
Wright's Stain is a mixture of methylene blue and eosin in methanol. Gram's stain is crystal violet, iodine washed with acetone and proofed with a safranin dye to look for gram negative organism.
What is the mordant in a gram stain?
Gram's iodine stain is applied after the culture is stained with the primary stain. It acts as a mordant, fixing the primary stain to the cell wall while lending no additional colour to the cell (i.e. the mordant itself is not a stain). The mordant is only able to fix the stain to Gram-positive bacteria because of the characteristic thick, peptidoglycan coat that they possess. Because the mordant is not able to fix the stain to Gram-negative bacteria (who's coat have a different composition), the crystal violet stain will wash away from Gram-negative bacteria when the decolourizing agent is added.
