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Q: Is Ca2 and Mg2 in PBS affect Cell function?
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What is the element that binds to troponin?

Ca2+ , Mg2+ .


Why Calcium and magnesium free PBS?

What cell walls are you using? In mammalian cell culture the reason you would use Ca/Mg free PBS is to help remove them from the surface they are attached to. The adherens proteins that adherent cells use to stick to whatever they are stuck to requires Ca2+ and Mg2+. Therefore if you simply want to wash the cells you have to use a buffer WITH Ca2+ and Mg2+.


What is the average hardness of a bottle of water?

Total hardness=(Ca2+)*(combining weight of CaCO3/combining weight of Ca2+) +(Mg2+)(combining weight of CaCO3/combining weight of Mg2+)


How does borax serves as water softening agent?

When Borax is added to water, it reacts with Ca2+ and Mg2+, which come out of water as precipitates. The reactions involved are: Ca2+ + Na2B4O7 ----> CaB4O7 + 2Na+ Mg2+ + Na2B4O7 -----> MgB4O7 + 2Na+


How does borax serve as a water softening agent?

When Borax is added to water, it reacts with Ca2+ and Mg2+, which come out of water as precipitates. The reactions involved are: Ca2+ + Na2B4O7 ----> CaB4O7 + 2Na+ Mg2+ + Na2B4O7 -----> MgB4O7 + 2Na+


What are cations?

Cations are positively charged ions. •Na+ •K+ •Ca2+ •Mg2+


What two elments have ions with a 2 charge?

Mg2+ and Ca2+ (and many more!)


What is the chemical symbol of hard water?

CaCO3 calcium carbonate


Is ca plus 2 or mg plus 2 larger?

The Ca2+ ion is larger than the Mg2+ ion. The ionic radii are: 86pm for the Mg2+ and 114pm for the Ca2+. Besides the fact that the Ca2+ has more electrons this can be explained by the principle of electron shielding. Electron shielding is when lower level electrons block the EFC(effective nuclear charge) from effecting the valence electrons of an ion. Ca2+has more electrons than Mg2+ so more electron shielding occurs.


Ca2 plus ions separate from Mg2 plus ions?

they cannot bond together, they are separate .


What are the major elements found in water?

•Na+, K+, Mg2+, Ca2+, SO42-, Cl-, NO3-, HCO3-


What is Dolomitization?

•2CaCO3 + Mg2+ > CaMg(CO3)2 + Ca2+ • •Fluid flow seems to control precipitation of dolomite