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The best way to relocate a lost object when using a high-power microscope is to raise the body tube, check the slide to make sure the object is still on it, and adjust the light source if necessary. Then, starting with the coarse adjustment knob start focusing on the object again.
In a sentence using the "three persons objective" point of view, the subject of the sentence is acting upon the object. For example: "She saw him." Here, "she" is the subject acting upon "him," which is the object.
yes it is.
Begin by using the lowest power objective lens and then switching to the next highest power lens.
When using a microscope, the initial focusing of any slide is done under either the 4x or 10x objective. Once you have your subject in focus, you may make further adjustments.
The fine adjustment knob is used with the power objective on the microscope. Since the objective is already at the highest objective possible for that microscope, using the coarse adjustment knob would focus to much on the object making the details harder to read. Using the fine adjustment knob, you are able to see the fine details within the object or organism you are studying. The higher the power, the finer the knob and the lower the objective, the coarser the knob and objective.
You use the low power objective lens first to get your sample centered in the field of view.
If you move closer while looking thru the lens, there is a danger of cracking the slide, especially using coarse adjustment.
the naswe is 400
450x TM ( magnification of the ocular lens ( 10x) multiplied by the magnification from the objective lens ( 45x)= 450x TM ( total magnification)
The total magnification would be 200x, since the total magnification is the magnification of the objective lens X the magnification of the eyepiece.
Not all microscopes have the same magnification for the lower power lens.