0
A column is said to be endcapped when a small silylating agent, such as trimethylchlorosilane, is used to bond residual silanol groups (-OH) on a packing surface. It is most often used with reversed-phase packings and may cut down on undesirable adsorption of basic or ionic compounds.
Wiki User
∙ 12y ago
Wiki User
∙ 13y agoA column is said to be endcapped when a small silylating agent, such as trimethylchlorosilane, is used to bond residual silanol groups on a packing surface. It is most often used with reversed-phase packings and may cut down on undesirable adsorption of basic or ionic compounds
Wiki User
∙ 14y agoThe reaction is referred to as end capping or end blocking. The result is that carbanion or carbocation centers do not form and depolymerization does no occur at the ceiling temperature.
Wiki User
∙ 12y agowhat is endcapping of hplc column
Add your answer:
Earn +20 pts
How does hplc works?
HPLC works when a reservoir holds the solvent and then it is sent to the pump manager.Next it goes to the HPLC coloumn .After it goes through there it usually ends in the detector than waste. Generally the stationary phase in the HPLC column is made up of alkyl coated silica making it relatively non-polar. Due to this the technique is also called reversed-phase HPLC.
What is the first step of gene splicing?
5 prime end capping
How fractionating column made?
why does temprature fall down toward the end of the fractional column
How do you separate the enzymes in column chromatography?
In column chromatography, the enzymes are made to pass through the column without occurrence of bubbles. These enzymes are obtained at the end of the process by slowly advancing through every column.
What is the need of capping of TMV RNA?
A cap is associated to the 5' end of mRNAs of TMV transcribed by PolyII , which is essential for recruiting translation initiating factor and for anchoring RNA of the actin/endoplasmic reticulum (ER) network.
What is the difference ods and ods2 HPLC Column?
BDS - Base deactivated Silanol ODS - Octadecyl silane by using the BDS column, residual silanols deacivated and silanol activity reduced, it is end-capped column and it is good for basic compounds. ODS is normal column and high acidic silica, it is not suitable for basic compounds.
How does hplc works?
HPLC works when a reservoir holds the solvent and then it is sent to the pump manager.Next it goes to the HPLC coloumn .After it goes through there it usually ends in the detector than waste. Generally the stationary phase in the HPLC column is made up of alkyl coated silica making it relatively non-polar. Due to this the technique is also called reversed-phase HPLC.
What is the first step of gene splicing?
5 prime end capping
How do you recap a needle?
Re-capping - is simply replacing the plastic protective cover over the end until it locks in place.
What is the silent in column?
I'm not sure what you mean by "silent in column." Can you please provide more context or clarify your question?
How fractionating column made?
why does temprature fall down toward the end of the fractional column
What protects mRNA from attack by cellular enzymes?
An eukaryotic mRNA has 2 ends, a 3' (three prime) end and a 5' (five prime) end. They are both protected from degradation. The 3' end is protecting by a long tail of the Adenosine base, this tail is reffered to as the Poly-A tail and is established through the process of polyadenylation. The 5' end has a different method of protection from degradation, it undergoes "capping". Capping involves a Gaunine base paring in a 5' - 5' manner with the exposed 5' end of the mRNA. This basically leaves no exposed 5'. An eukaryotic mRNA has 2 ends, a 3' (three prime) end and a 5' (five prime) end. They are both protected from degradation. The 3' end is protecting by a long tail of the Adenine base, this tail is reffered to as the Poly-A tail and is established through the process of polyadenylation. The 5' end has a different method of protection from degradation, it undergoes "capping". Capping involves a Gaunine base paring in a 5' - 5' manner with the exposed 5' end of the mRNA. This basically leaves no exposed 5'.
What is a batch distilation column?
This is a distillation column that doesn't have a continuous feed. The material that is to be distilled will typically be in a round bottom still at the end of the column.
What does bottom of the first column mean?
Go to the first column (defind by the context of the question) and scroll down until you find the end of the column.
What is at the end of the vertebral column?
Skull at one end, tail bone at the other.
What column contains elements whose electron configuration end with d4?
what column of the periodic table contains elements whose electron configurations end with d4
What is an artificial end to a column to balance columns or to provide space for the insertion of other objects?
manual column break
