heat fixing your slide causes the cells to stick to the slide, without that the cells will be washed right off and there will be nothing left to observe.
Fontana silver staining.
The bacterial smear will wash away during the staining procedure. This is avoided by heat fixation, during which the bacterial proteins are coagulated and fixed to the glass surface.
Carbohydrate demonstration methods. Demonstrates Staining mechanisms and technical comments.
Gram, Ziel-Nielsen
flagellar stain
This is simply important in order to have accurate staining results. If this is not followed, the process of the staining will result to false positives or false negatives.
acid fast staining or Ziel Neelson staining for observing Mycobacteria tuberculosis or Koch bacilli from sputum sample.
Fluorescein staining
fluorescein staining
there is no real effect
capsule does not gets stained it appears to be colourless when stained using manewals staining procedure
In Gram staining procedure during bacterial staining , iodine forms a complex with crystal violet stain which stains Gram positive bacteria blue to violet .