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They help you eliminate non-specific products. That is, you'll reduce the number of chains of inappropriate lengths you generate. You should see just one solid mark on your gel.
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Why do you use a negative control in PCR?
to check is there any contamination in pcr products
Use of water in pcr?
enzyme cofactor
What is a Taqman Real Time PCR?
One can use a PCR to amplify and quantify a certain DNA molecule. A TaqMan real-time PCR is a certain type of PCR which uses the TaqMan method to increase its specificity.
What is the purpose of mgcl in pcr?
The enzyme DNA polymerase ( Taq polymerase) used in the PCR requires Mg 2+ ions for its functioning.These Ions act as cofactors for the enzyme . Hence the requirement for the use of Mg Cl2 in PCR reactions.
Why use touchdown PCR?
We use this principe when the annealing temperature of the two primers (reverse and forward) is different
Is francium a cation or anion?
francium can form monovalent cations.
What is the formulas of lithium hydride what is the answer for the formula of lithium hydride?
LiH. Lithium forms monovalent cations, and in the presence of a very low electronegativity metal such as lithium, hydrogen forms monovalent anions.
What is the use of dNTP?
The use of dNTP is PCR and multiplex PCR
Predict what the formula would be for a compound formed between potassium and sulphur?
K2S. Potassium forms monovalent cations and sulphur forms divalent anions.
What characteristics does group 1 share from the periodic table?
Group 1contain alkali metals; all are very reactive, react also with water, form monovalent cations.
What are the major characteristics of the metals in group one?
These metals have low density, they are extremely reactive, they are monovalent, the electronegativity is low, they react violent with water etc.Group-1 metals have 1 valence electron. They form mono cations.
What is the binary ionic compound of magnesium and chlorine?
Magnesium forms divalent cations and chlorine forms monovalent anions; therefore, the binary compound of magnesium and chloride has the formula MgCl2.
Why do you use a negative control in PCR?
to check is there any contamination in pcr products
Will MgF3 form based on the charge of their ions?
No: Magnesium forms divalent cations, while fluorine forms monovalent anions. The compound between these two elements will therefore have the formula MgF2.
Predict the empirical formula of the ionic compound that forms from magnesium and fluorine?
Magnesium usually forms divalent cations and fluorine usually forms monovalent anions. Therefore, the predicted empirical formula is MgF2.
Use of water in pcr?
enzyme cofactor
What are the different types of polymerase chain reaction techniques?
types of pcr: AFLP -PCR. Allele-specific PCR. Alu-PCR. Assembly -PCR. Assemetric -PCR. Colony -PCR. Helicase dependent amplification. Hot start pCR. Inverse -PCR. Insitu -pCR. ISSR-PCR. RT-PCR(REVERSE TARNSCRIPTASE). REAL TIME -PCR
