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WHY IS NITROGEN SUPPLIED AS AMMONIUM SALTS (SPECIFICALLY) RATHER THAN AS AMINO ACIDS IN SIMMONS CITRATE AGAR?
Wiki User
∙ 8y ago
If amino acids were used they would not produce the same degree of pH change that results in color change of the indicator.
Wiki User
∙ 8y ago
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Why is the citrate test carried out on an agar slope rather than a broth?
Background:Simmons' Citrate Agar is a defined, selective medium that tests for an organism's ability to use citrate as a sole carbon source and ammonium ions as the sole nitrogen source.The medium contains citrate, ammonium ions, and other inorganic ions needed for growth.It also contains bromothymol blue, a pH indicator. Bromothymol blue is green at pH below 6.9, and then turns blue at a pH of 7.6 or greater.SO:Sometimes, it is possible to detect growth on the citrate slant without the accompanying color change to blue. This is most likely due to insufficient incubation. Either a combination of blue color and growth or growth alone without the blue color should be scored as a positive for the citrate use test.
What are some uses of ammonium citrate?
Ammonium Citrate in distilled water is an alternate developer for Platinum and Palladium photographic prints (Iron replacement by Pt and/or Pd). It is cooler in resulting print color than the usual developer - Potassium Oxalate. See www.bostick-sullivan.com
How does klebsiella pneumoniae use citrate as a source of carbon?
Klebsiella pneumoniae is a member of the Enterobacteriaceae family and is able to anaerobically use citrate as a source of Carbon because the citrate contains citritase, an enzyme that initiates a catabolic process. The carbonate and nitrogen of the citrate is broken down into acetate and oxalacetic acid, and then pyruvic acid and carbon dioxide. These products reacts with the ammonia (nitrogen and hydrogen) and sodium hydrogen carbonate compounds of the Citrate. The K. pneumoniaeaccumulates the alkalines and create a basic pH. (I think?)
What are the 10 most common salts?
Examples are: sodium chloride, potassium chloride, calcium chloride, ammonium chloride, ammonium phosphates, sodium carbonate, sodium sulfate, magnesium sulfate, cooper sulfate, magnesium chloride.
PH of potassium citrate?
Potassium citrate is an alkaline salt.
What is the chemical formula of ammonium citrate?
Ammonium citrate formula is: C6H14N2O7.
Ferrous fumarate ferric ammonium citrate iron dextrose?
how can i prepare ferric ammonium citrate redish crystals
Home come E.coli can't grow on Simmons Citrate Media?
Because it cannot utilize citrate as the sole carbon source and/or they cannot utilize ammonium ions as the sole nitrogen source.
How do you make Ammonium ferric citrate?
ferric hydroxide + citric acid ---> product A (ferric citrate)Product A + ammonia solution --> ammonium ferric citratecheck out for stoichiometric equation
Can you make ammonium citrate from citric acid and ammonium hydroxide?
Yes H2C2O4 + 2NH3 --> (NH4)2C2O4
How much elemental iron in 100 mg ferric ammonium citrate?
18mg
What is the molar mass of ferric ammonium citrate?
mole mole mole mole mole
Why is the citrate test carried out on an agar slope rather than a broth?
Background:Simmons' Citrate Agar is a defined, selective medium that tests for an organism's ability to use citrate as a sole carbon source and ammonium ions as the sole nitrogen source.The medium contains citrate, ammonium ions, and other inorganic ions needed for growth.It also contains bromothymol blue, a pH indicator. Bromothymol blue is green at pH below 6.9, and then turns blue at a pH of 7.6 or greater.SO:Sometimes, it is possible to detect growth on the citrate slant without the accompanying color change to blue. This is most likely due to insufficient incubation. Either a combination of blue color and growth or growth alone without the blue color should be scored as a positive for the citrate use test.
What happens when you mix citric acid baking soda and vinegar?
A mixture of ammonium acetate and citrate is obtained.
Why is ammonium in soft drinks?
Ammonium ferric citrate is a food additive with E number E381 used as an acidity regulator. It is a green or reddish-brown powder which is very soluble in water.
Does irn bru have iron in it?
Yes, albeit a very minuscule amount (less than 0.002%), in the form of ammonium ferric citrate.
What are some uses of ammonium citrate?
Ammonium Citrate in distilled water is an alternate developer for Platinum and Palladium photographic prints (Iron replacement by Pt and/or Pd). It is cooler in resulting print color than the usual developer - Potassium Oxalate. See www.bostick-sullivan.com
