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How does klebsiella pneumoniae use citrate as a source of carbon?
Wiki User
∙ 13y ago
Klebsiella pneumoniae is a member of the Enterobacteriaceae family and is able to anaerobically use citrate as a source of Carbon because the citrate contains citritase, an enzyme that initiates a catabolic process. The carbonate and nitrogen of the citrate is broken down into acetate and oxalacetic acid, and then pyruvic acid and carbon dioxide. These products reacts with the ammonia (nitrogen and hydrogen) and sodium hydrogen carbonate compounds of the Citrate. The K. pneumoniaeaccumulates the alkalines and create a basic pH. (I think?)
Wiki User
∙ 13y ago
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What does the citrate utilization test determine?
this test is used to identify bacteria which use citrate as sole source of carbon e.g klebsila, pseudomonas aerogenosa
What does the citrate metabolic test test for?
it tests the ability of a bacteria to use citrate as its sole carbon source. if its positive, it will raise the pH and there will be no acid in the end product.
What is the result of citrate test for bacillus licheniformis?
The result is the initial green color of the Simmons Citrate Agar turning blue; generating a positive indication that the organism does indeed utilize citrate as the sole source of carbon and energy.
Does pseudomonas aeruginosa have a positive result for citrate utilization?
The color changes from the slant's original green to blue, indicationg that the species is able to utilize citrate as a carbon source. Thus, it is POSITIVE
Is the source of sodium citrate natural?
Yes, the source of sodium citrate is natural. Sodium citrate occurs naturally in citrus fruits such as pineapples, lemons and oranges.
Citrobacter freundii utilize citrate?
Yes, Citrobacter freundii can metabolize lactose or citrate as a carbon source.
What does the citrate utilization test determine?
this test is used to identify bacteria which use citrate as sole source of carbon e.g klebsila, pseudomonas aerogenosa
Home come E.coli can't grow on Simmons Citrate Media?
Because it cannot utilize citrate as the sole carbon source and/or they cannot utilize ammonium ions as the sole nitrogen source.
What does the citrate metabolic test test for?
it tests the ability of a bacteria to use citrate as its sole carbon source. if its positive, it will raise the pH and there will be no acid in the end product.
What is the result of citrate test for bacillus licheniformis?
The result is the initial green color of the Simmons Citrate Agar turning blue; generating a positive indication that the organism does indeed utilize citrate as the sole source of carbon and energy.
Does pseudomonas aeruginosa have a positive result for citrate utilization?
The color changes from the slant's original green to blue, indicationg that the species is able to utilize citrate as a carbon source. Thus, it is POSITIVE
Is the source of sodium citrate natural?
Yes, the source of sodium citrate is natural. Sodium citrate occurs naturally in citrus fruits such as pineapples, lemons and oranges.
Citrate utilization test?
The test indicates if an organism utilizes Citrate as it's only source of Carbon. Positive results will show growth on slant as well as changing the medium from green to blue (ph indicator)
Account for the development of alkalinity in cultures capable of using citrate as their sole carbon source?
Citrate is acted on by the enzyme citrase, which produces oxaloacetic acid and acetate. These products are then enzymatically converted to pyruvic acid and carbon dioxide. During this reaction the meduim becomes alkaline (the carbon dioxide that is generated combines with sodium and water to form sodium carbonate, and alkaline product.
What is simmons citrate agar?
Simmons citrate agar is a differential agar used to determine if a sample bacteria can utilize citrate as its only carbon source. The agar is initially a green color due to the bromo thymol blue pH indicator in it. If a bacteria uses the citrate, the by-products are ammonia and ammonium hydroxide both of which will alkalize the agar and increase the pH to the point of changing the indicator's color to blue, so the whole agar turns from green to blue.
What is the color of mac conkey agar of klebsiella?
It's pink, since it can use lactose as it's nutrition source
What is a citrase?
In microbiology... I believe it is an enzyme in some bacteria that metabolize Simmon's citrate as their sole carbon source, and not sugar. it's used for identification among bacteria. If it has this ability, it can release ammonioa and increase the pH of the medium. A color change to blue indicates it is citrate positive. anything in science that ends in "ase" is an enzyme.
