The result is the initial green color of the Simmons Citrate Agar turning blue; generating a positive indication that the organism does indeed utilize citrate as the sole source of carbon and energy.
blue/positive
No, it is present/positive. • Cocci* • Gram-Positive* • Non-motile* • No Capsules* • No Spores* • Facultative Aerobe* • No Glucose, Lactose*, Mannitol* Fermentation • No Urease*, Catalase*, Oxidase*, Ligase*, Protease* Production • Production of Indole*, tryptophonase*
Bacillus subtillus will test positive when performing a VP and Citrate test. Also, bacillus cereus is much more motile. It isn't as dependable as the chemical tests, but the cereus colonies will appear wispy and more widespread on an agar plate than those of subtilis.
Bacillus megaterium is catalase-positive, meaning it produces the enzyme catalase which breaks down hydrogen peroxide into water and oxygen. In a catalase test, if Bacillus megaterium is added to hydrogen peroxide, you would observe the formation of bubbles or effervescence due to the release of oxygen gas. This is a positive catalase test result for Bacillus megaterium.
Yes, Serratia marcescens typically tests negative for citrate utilization in citrate utilization tests. This bacterium lacks the enzyme citrate-permease needed to import citrate into the cell, resulting in a negative citrate test.
blue/positive
The B.cereus has a positive citrate test and a negative Voges Proskauer.
Most bacteria in Tribe V are able to utilize Citrate, with the exception of Hafnia and Pantoea (+/-). The 2 most common Klebsiella (oxytoca and pneumoniae), can utilize citrate, so should be citrate positive.
Pseudomonas aeruginosa typically demonstrates a negative result on the citrate test, as it is unable to utilize citrate as its sole carbon source for growth. This bacterium lacks the enzyme citrate-permease needed to transport citrate into the cell for metabolism, leading to a lack of growth on citrate agar and a lack of color change from green to blue.
The positive organism for citrate utilization test is usually Escherichia coli. When this bacterium is able to grow on a citrate-containing medium, it will produce alkaline byproducts that change the pH of the medium, turning it from green to blue. This color change indicates a positive result for citrate utilization.
bacillus megatarium test positive for sucrose where creus doesnt
No, it is present/positive. • Cocci* • Gram-Positive* • Non-motile* • No Capsules* • No Spores* • Facultative Aerobe* • No Glucose, Lactose*, Mannitol* Fermentation • No Urease*, Catalase*, Oxidase*, Ligase*, Protease* Production • Production of Indole*, tryptophonase*
Bacillus subtillus will test positive when performing a VP and Citrate test. Also, bacillus cereus is much more motile. It isn't as dependable as the chemical tests, but the cereus colonies will appear wispy and more widespread on an agar plate than those of subtilis.
Bacillus megaterium is catalase-positive, meaning it produces the enzyme catalase which breaks down hydrogen peroxide into water and oxygen. In a catalase test, if Bacillus megaterium is added to hydrogen peroxide, you would observe the formation of bubbles or effervescence due to the release of oxygen gas. This is a positive catalase test result for Bacillus megaterium.
Yes, Serratia marcescens typically tests negative for citrate utilization in citrate utilization tests. This bacterium lacks the enzyme citrate-permease needed to import citrate into the cell, resulting in a negative citrate test.
Based on my own test, Shigella flexneri tests negative for the citrate test.
assay test of sodium acid citrate