The result is the initial green color of the Simmons Citrate Agar turning blue; generating a positive indication that the organism does indeed utilize citrate as the sole source of carbon and energy.
blue/positive
casein hydrolysis
Bacillus subtillus will test positive when performing a VP and Citrate test. Also, bacillus cereus is much more motile. It isn't as dependable as the chemical tests, but the cereus colonies will appear wispy and more widespread on an agar plate than those of subtilis.
No, Serratia and Klebsiella test positive for citrate. E.Coli tests negative.
M. Morganii is citrate negative
blue/positive
The B.cereus has a positive citrate test and a negative Voges Proskauer.
citrate result is positive in case of pseudomonas aeroginosa. slant and butt both will be positive
casein hydrolysis
bacillus megatarium test positive for sucrose where creus doesnt
Staphylococcus Epidermidis does return a gram positive result on the citrate lab test. Gram stains will produce these results on slides.
Bacillus subtillus will test positive when performing a VP and Citrate test. Also, bacillus cereus is much more motile. It isn't as dependable as the chemical tests, but the cereus colonies will appear wispy and more widespread on an agar plate than those of subtilis.
Based on my own test, Shigella flexneri tests negative for the citrate test.
Citrate Permease is an enzyme that is responsible for transporting citrate inside the cell. Some bacteria have this enzyme while others do not. You can test for the presence of citrate permease by performing a Simmon's Citrate biochemical test.
assay test of sodium acid citrate
The positive test control organism for citrate utilization test is Klebsiella pneumoniae
No, Serratia and Klebsiella test positive for citrate. E.Coli tests negative.