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1. What is the purpose of inverting inoculated plates during incubation?2. Where should colonies appear in the case of : a. Streak plate b. pour plates3. Indicate the temperature ranges for the following microbial categories.a. psychropiles b. mesophiles c. thermopiles4. What factors could account for an absence of growth on a pour plate?5. What factors could account for an absence of growth on a streak plate?6. What explanations could be given for the failure of obtaining isolated colonies on a streak plate?7. Gelatin and Agar comparison:a. Chemical composition b. temperature required for melting and solidifyc. Possibility of enzymatic attack by bacteria, Yes or No.
Previous answer: "because of infection" This person is obviously trying to be funny, the right word should be "Contamination", as for spread plate, the bacteria is more exposed to air as it is spread over the agar plate. Therefore, the result might not be accurate, as it might be contaminated. As for the pour plate method, the bacteria is in the agar itself, it is not exposed to air, thus, less risk of getting contaminated.
The purpose of a pour plate is to exam the bacteria in milk. It is used to find isolated bacteria colonies under anaerobic and aerobic environments.
When we have to isolate a specific microbial species from their mix culture or to grow any microbe on solid surface for their further studies then they can be grow on a culture medium containing a gel like substance known as agar which produce disticnt microbial colonies when inoculate in a petri dish containing the growth medium. The way by which the inoculation of microbial sample done is called a streak plate method in which the microbial sample is streak with the help of inoculating loop on the agar plate firmly, in the way so that the cell can be isolated. There are two more method to incoulate the microbial sample that are: pour plate and spread plate techniques.
It is necessary to make the colonies well-isolated from each other so that each appears distinct, large and shows characteristic growth forms.Most bacteria, many other microfungi, and unicellular microalgae, may be most commonly obtained by plating methods such as streak plate method, pour plate method and spread plate method.
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Put simply - yes. Some strictly aerobic organisms will not grow in a pour plate. They may, however proliferate on a streak plate. Also consider the posibility of experimental error. The culture may have been added to the molten agar when it was too hot for the organisms to survive.
The streak plate method makes it easier for colonies of bacteria to grow. It also generally leads to individual colonies that look like small dots, rather then simply a mat of bacterial growth.
The streak plate method makes it easier for colonies of bacteria to grow. It also generally leads to individual colonies that look like small dots, rather then simply a mat of bacterial growth.
It is more likely to give individual colonies regardless of the concentration of the original source. With pour plates, you might have to use several plates with different dilutions of inoculum to get individual colonies.
Colonies growing on a pour plate have slightly less avalible oxygen and are confined by the gel matrix so they tend to grow smaller than those on a pour plate. Streak plates are use to isolate single colonies, pour plates are used to enumerate batceria.
In the pour plate, the microorganisms will grow within the gel that has been set, and in the spread-plate technique, growth will be on top of the agar gel where it has been spread.
1. What is the purpose of inverting inoculated plates during incubation?2. Where should colonies appear in the case of : a. Streak plate b. pour plates3. Indicate the temperature ranges for the following microbial categories.a. psychropiles b. mesophiles c. thermopiles4. What factors could account for an absence of growth on a pour plate?5. What factors could account for an absence of growth on a streak plate?6. What explanations could be given for the failure of obtaining isolated colonies on a streak plate?7. Gelatin and Agar comparison:a. Chemical composition b. temperature required for melting and solidifyc. Possibility of enzymatic attack by bacteria, Yes or No.
Previous answer: "because of infection" This person is obviously trying to be funny, the right word should be "Contamination", as for spread plate, the bacteria is more exposed to air as it is spread over the agar plate. Therefore, the result might not be accurate, as it might be contaminated. As for the pour plate method, the bacteria is in the agar itself, it is not exposed to air, thus, less risk of getting contaminated.
A disadvantage of the streak plate technique could be colony isolation problems. If the streaking technique is not done properly or if there is too much of an organism present on the inoculating loop then the cells can cluster and form what looks like one colony but it can actually be a couple colonies (made from a couple cells). This can cause an inaccurate colony forming unit count.
1 The Spread Plate: If a mixture of cells is spread out on an agar surface so that every cell grows into a completely separate colony, a macroscopically visible growth or cluster of microorganisms on a solid medium, each colony represents a pure culture. The spread plate is an easy, direct way of achieving this 2 The Pour Plate: Extensively used with bacteria and fungi, a pour plate also can yield isolated colonies. The original sample is diluted several times to reduce the microbial population sufficiently to obtain separate colonies when plating result. 3 The streak plate: Pure colonies also can be obtained from streak plates. The microbial mixture is transferred to the edge of an agar plate with an inoculating loop or swab and then streaked out over the surface in several patterns
You can identify a contamination on a pour plate by color, size, shape, texture, or growth rate.