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it is a labours process
it is timeconsuming process
it required more amount of dna require 3microliter dna per sampel
it is costly
Wiki User
∙ 11y ago
Wiki User
∙ 14y agoto get expressed proteins.
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Is there a replacement for blotting paper?
A blotting paper is used to absorb excess oil from your skin.
What is the color of the queens blotting paper?
I heard that The Queen uses black blotting paper in order that no one could read what she had written. I wrote to her to ascertain the veracity of this practice and received the following return from Miss Michele Edwards who is an Information Officer in Her Majesty's Press Office. Thank you for your letter of 22nd March. "The rumour about The Queen using black blotting paper seems to surface fairly regularly. There is no truth in this; Her Majesty uses ordinary bloting paper." ... more, not relevant. Yours sincerely etc.etc. I hope this answers you query.
What are the disadvantages for disadvantages?
gramophone
What are the disadvantages and disadvantages of object oriented DBMS?
#
What are the disadvantages of mining iron?
The disadvantages of mining iron
Is etbr transfer with DNA during southern blotting?
The answer is NO! To me, it makes no sense, why we have to transfer etbr with DNA to a blotting membrane? When Southern blotting signals will be detected by means of Radiochemicals or fluro labelling why we have to think about EtBr?
Difference between southern and northern blotting?
Southern Blotting refers to the identification of detailed sequences of DNA in which the DNA fragments are separated by electrophoresisNorthern Blotting refers to the identification of detailed sequences of RNA in which the RNA fragments are separated by electrophoresis
What are some other techniques that build on the Southern Blotting technique?
Certain forensic applications, such as using blood samples to determine the killer of a victim, rely on Southern blotting.
Is the principle of northern southern and western blotting is same?
yes the principle of all the three is same .the basic difference lies during probing nd detection .in case of southern blotting DNA is used in nothern rna and in case of western blotting antibodies are ued
Which describes the transfer of mRNAs to a membrane to analyze gene expression?
southern blotting
What is blotting and types of blotting?
Blotting is a technique of transferring DNA or RNA or Protein from a gel to the membrane. nitrocellulose, PVDF or nylon membrane are used to attach these molecules permanently on them. Western, southern and norther blotting are the techniques used to transfer protein, DNA and RNA respectively. It is done to detect them with a specific probe or antibody. This can not be detected or easy to handle on the gel, so we do blot for these analysis.
What is a blad?
A blad is a Scots term for a portfolio, blotting-book or blotting-pad.
Can chlamydia cause blotting?
Blotting? As In Bleeding, then yeah It doesYou can bleed from being infected by chlamydia.
What is the theory behind southern blotting?
Southern Blotting Is a method used in molecular biology for the detection of a specific DNA order in when testing DNA samples. The method was named after Edward M. Southern who developed the procedure in the 1970s.
What is blotting technique?
hide
How do you do western blotting from RNA?
A western blot cannot be prepared from RNA. Only a protein sample can be run with a western blotting approach. The technique used to visualize RNA on a gel is called northern blotting.
What were the advantages and disadvantages of building more towns in the southern colonies?
Colonists
