urease negative
proteus mirabilis
Coliforms are typically gram-negative bacteria, characterized by their ability to ferment lactose. However, some coliform bacteria can exhibit varying characteristics, including gram-positive features.
in the absence of glucose or lactose , some bacteria are able to use citrate as only source of carcon. alkaline pH change the bromothymol blue indicator from green to blue. pseudomonas can not ferment the glucose and the lactose so it use the citrate as carbon source.
Negative lactose fermentative organisms are bacterial species that do not ferment lactose, a type of sugar. This means they are unable to utilize lactose as a carbon source for energy production. Examples include Salmonella and Shigella species.
Lactose fermentation is common among enteric gram negative bacteria because they often inhabit the gastrointestinal tract where lactose is a common sugar present in the diet. These bacteria have evolved the ability to ferment lactose as a source of energy, allowing them to thrive in the intestinal environment. Fermentation of lactose also produces acid and gas, which can be used for diagnostic purposes to identify certain bacterial species.
No, it doesn't. After performing the Lactose fermentation test, the isolated colonies will turn yellow meaning that the test was negative.
bacillus subtilis
The positive control of the lactose operon in bacteria involves the binding of the catabolite activator protein (CAP) to the CAP site upstream of the promoter, enhancing RNA polymerase binding and increasing transcription of the operon in the presence of glucose limitation. This allows the bacteria to effectively utilize lactose as an alternative carbon source when glucose is scarce.
Negative lactose fermentative organisms are bacterial species that do not ferment lactose, a type of sugar. This means they are unable to utilize lactose as a carbon source for energy production. Examples include Salmonella and Shigella species.
in the absence of glucose or lactose , some bacteria are able to use citrate as only source of carcon. alkaline pH change the bromothymol blue indicator from green to blue. pseudomonas can not ferment the glucose and the lactose so it use the citrate as carbon source.
Macconkey agar consists of agar, lactose, peptone, neutral red dye, crystal violet dye & bile salts. Bile salts inhibit growth of Gram positive bacteria & allow growth of Gram negative bacteria only. It differentiates lactose fermentating Gram negative bacteria from non lactose fermentating Gram negative bacteria.
Lactose fermentation is common among enteric gram negative bacteria because they often inhabit the gastrointestinal tract where lactose is a common sugar present in the diet. These bacteria have evolved the ability to ferment lactose as a source of energy, allowing them to thrive in the intestinal environment. Fermentation of lactose also produces acid and gas, which can be used for diagnostic purposes to identify certain bacterial species.
No, it doesn't. After performing the Lactose fermentation test, the isolated colonies will turn yellow meaning that the test was negative.
In the ONPG test, organisms may be positive due to the presence of beta-galactosidase enzyme, which cleaves ONPG and produces a yellow color. However, in the lactose fermentation test, some organisms may lack other necessary enzymes or transport systems to fully metabolize lactose, resulting in a negative result despite having beta-galactosidase.
EMB is an undefined selective/differential medium. It contains aniline dyes (methylene blue and eosin), which inhibit the growth of Gram-positive bacteria selecting for Gram-negative bacteria. EMB also contains lactose which makes the media differential based on an organisms ability to ferment lactose.
McConkey agar is a selective and differential culture medium used in microbiology to isolate and differentiate lactose-fermenting Gram-negative bacteria such as Escherichia coli from other Enterobacteriaceae. It contains bile salts and crystal violet that inhibit the growth of Gram-positive bacteria, and lactose and neutral red that allow differentiation of lactose fermenters based on pH changes in the media.
EMB (Eosin Methylene Blue) is a defined medium because the composition and exact amounts of each ingredient are known. This formulation is desirable for consistent and reproducible results in microbial culture experiments because researchers can control and understand the nutritional content of the medium.
B. subtilis does not ferment glucose nor lactose