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Q: What can you conclude about the relationship between toxin secretion and culture density in S. aureus?
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Why are contrasting colors important in gram staining?

# Suppose you performed a gram stain on a sample from a pure culture of bacteria and observed a friend of red and purple cocci. Adjacent were not always the same color. What do you conclude? # ## The bacteria was not a pure culture.


Could you distinguish between axenic culture and pure culture?

a pure culture is the one that originate from a single cell and has same specie of an organism while axenic culture can originate from more than one cell.


Would it be useful to perform a gram stain on a mixed culture?

The purity of a culture of bacteria is important so it can test on that one type of bacteria. Gram staining can be good so you make sure everything in the streak plate is one color showing that it is gram positive and gram negative.


What is the difference between pure culture and auxanic culture?

A pure culture is one in which only one kind of microbial species is found whereas in mixed culture two or more microbial species formed colonies.


If an inoculated plate had colonies between the streak lines what would you conclude?

It depends, if there is no growth or colony appearance on streak line and only it shows growth in b/w the streak line then it is certainly a contamination and if there are colonies on streak line and not ressemble with the streak culture then also it is a contamination but there can be a chance that colony appears due to some fault in streaking procedure and the inoculum drops between the streak line so it depends.

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