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What charge is carried by the pigments in electrophoresis separation?
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What charge is carried by the dye pigment in the separation?
h
Why the pH does affect the separation of amino acids by electrophoresis?
The separation of amino acids by electrophoresis is influenced by the pH because it affects the ionization state of the amino acids. At different pH levels, amino acids can exist in different forms (protonated or deprotonated), which affects their charge. Amino acids with different charges will migrate at different speeds during electrophoresis, leading to their separation.
Which features of DNA fragments are used to separate them in the process of gel electrophoresis?
The separation is caused by the electrical direct current applied to the gel. Those molecules charged negatively will tend to go to the anode (positive) and those negatively charged migrate to the cathode.
What are the similarities between paper chromatography and electrophoresis?
Chromatography is a collective term for a family of lab techniques for the separation of the mixtures.It involves a passing a mixture dissolved in a mobile phase through a stationary phase. Electrophoresis is the process by which molecules (such as proteins, DNA, or RNA fragments) can be separated according to size and electrical charge by applying an electric current to them. Each kind of molecule travels through the medium at a different rate, depending on its electrical charge and molecular size In the electrophoresis techniques electricity is required and positive charge goes to the cathode whereas the negative charges goes to the anode (opposite charges attraction) but in Chromatography there is no need for the current or electricity .
Separate plasma proteins by electrical charge?
electrophoresis
What charge is carried by the dye pigment in the separation?
h
Why the pH does affect the separation of amino acids by electrophoresis?
The separation of amino acids by electrophoresis is influenced by the pH because it affects the ionization state of the amino acids. At different pH levels, amino acids can exist in different forms (protonated or deprotonated), which affects their charge. Amino acids with different charges will migrate at different speeds during electrophoresis, leading to their separation.
Which features of DNA fragments are used to separate them in the process of gel electrophoresis?
The separation is caused by the electrical direct current applied to the gel. Those molecules charged negatively will tend to go to the anode (positive) and those negatively charged migrate to the cathode.
Separate plasma proteins by electrical charge?
electrophoresis
What are the similarities between paper chromatography and electrophoresis?
Chromatography is a collective term for a family of lab techniques for the separation of the mixtures.It involves a passing a mixture dissolved in a mobile phase through a stationary phase. Electrophoresis is the process by which molecules (such as proteins, DNA, or RNA fragments) can be separated according to size and electrical charge by applying an electric current to them. Each kind of molecule travels through the medium at a different rate, depending on its electrical charge and molecular size In the electrophoresis techniques electricity is required and positive charge goes to the cathode whereas the negative charges goes to the anode (opposite charges attraction) but in Chromatography there is no need for the current or electricity .
What is the principle of electrophoresis?
Electrophoresis works on the principle of migration of charged particles (toward their opposite charge) in the presence of electric field.
Some viva voce questions for electrophoresis?
1. WHAT IS ELECTROPHORESIS AND WHAT ARE THE IMPORTANTAPPLICATIONS OF ELECTROPHORESIS?Ans. Movement of charged particle in the electric field either towards cathode or anode whensubjected to an electric current is called electrophoresis.The following factors influence the movement of particles during the electrophoresis.(a) Electric current.(b) Net charge of the particle.(c) Size and shape of the particle.(d) Type of supporting media.(e) Buffer solution.Important Applications of ElectrophoresisThe technique of electrophoresis is used to separate and identify the(i) Serum proteins(ii) Serum lipoproteins(iii) Blood hemoglobins2. WHAT ARE THE DIFFERENT TYPES OF ELECTROPHORESIS?Ans. (a) Moving boundary electrophoresis: This technique was first introduced by TISELIUS in 1937(b) Zone electrophoresis: In this type of electrophoresis different types of supporting mediaare used. These are;(a) Paper electrophoresis(i) Whatman filter paper(ii) Cellulose acetate(b) Gel electrophoresis(i) Agarose.(ii) Polyacrylamide gel (used for the separation of isoenzymes).(iii) SDS-PAGE.(iv) Iso-electric focussing (proteins seperated in a medium possessing a stable pH gradient).(v) Immuno electrophoresis (for the separation of immunoglobulins).
Is electrophoresis part of biotechnology?
Electrophoresis is used to separate molecules based on size and charge. Since biotechnology depends on knowing what you are working with, electrophoresis of proteins, DNA and RNA is a tool used by biotechnologists.
For what purpose might one use capillary electrophoresis?
Capillary electrophoresis is a technique used in laboratories to separate molecules based on their charge in order to study and analyze them. Capillary electrophoresis uses an electric charge to force the movement of molecules since each molecule will go a varying distance based on the weight of the molecule and their charge. Some areas of study that use capillary electrophoresis include DNA sequencing and pharmaceutical analysis.
Method of separating out plasma proteins by electrical charge?
Electrophoresis
What separates molecules by movement due to size and electrical charge?
gel electrophoresis
What are the name of separating techniques involving electric charge on molecules?
Gel electrophoresis.
