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What color are bacterial endospores after a gram stain is performed?
Wiki User
∙ 14y ago
Gram-staining does not stain the endospore due to the tough, resistant water-proof structure. It appears as an unstained area in a vegetative cell. Malachite green must be forced into the endospore with heat to stain it.
Wiki User
∙ 14y ago
Wiki User
∙ 9y agoAfter gram staining an endospore the color it would show up would be colorless or clear. It will not work for endospores because of its tough outer layer, stains can't penetrate.
Wiki User
∙ 10y agoPurple
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What dye is used to stain endospores?
To differentiate the spores from other similar looking structures in the cell that don't stain as the spores do.
Assume you stain Bacillus by applying malachite green with heat and then counterstaining with safranin Through the microscope the green structures are?
The malachite green penetrates the endospores, the counterstain of safranin will stain the vegetative cells. Endospores - greenish-blue Vegetative Cells - Red/Pink
What causes a stain to adhere to bacterial cells?
In a gram positive stain it is because the cell's cell wall is made up of peptioglycan
What is the purpose of covering the smear with bibulous paper when performing the endospore stain?
This paper will aid in keeping the stain over the bacterial smear.
What does a negative result for a spore stain indicate about the organism?
A negative result for the spores stain indicate that the gram-negative organism is present. A positive result for a spore stain indicates that a gram positive organism is present.
What color do bacterial endospores stain in the Schaeffer-Fulton spore stain?
Green
Are endospores acid fast?
No, endospores are not acid fast because they are not cells. Endospores will stain with the acid fast stain and look fuchsia but they are not positive for acid fast.
What color are endospores after endospore stain?
After gram staining an endospore the color it would show up would be colorless or clear. It will not work for endospores because of its tough outer layer, stains can't penetrate.
What dye is used to stain endospores?
To differentiate the spores from other similar looking structures in the cell that don't stain as the spores do.
Assume you stain Bacillus by applying malachite green with heat and then counterstaining with safranin Through the microscope the green structures are?
The malachite green penetrates the endospores, the counterstain of safranin will stain the vegetative cells. Endospores - greenish-blue Vegetative Cells - Red/Pink
What does the gram stain in a cerebrospinal fluid analysis indicate?
The Gram stain is performed on a sediment of the CSF and is positive in at least 60% of cases of bacterial meningitis.
What color are gram negative cells?
Gram positive bacterial stain purple. They do not take up the counter stain.
Why heat the slide when performing spore stain?
Endospores are impermeable to most stains so heat is usually applied to drive the stain into the endospore.
Why gram staining procedure cannot stain endospores?
Because stains can't penetrate the spore covering. You have to heat the spore to open it and let the stain in.
What color is Gram- stain?
Gram- negative bacteria turns red to pink after the gram stain is performed.
What color is neg?
Gram- negative bacteria turns red to pink after the gram stain is performed.
What color is gram?
Gram- negative bacteria turns red to pink after the gram stain is performed.
