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Endospores are impermeable to most stains so heat is usually applied to drive the stain into the endospore.
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Why gram staining procedure cannot stain endospores?
Because stains can't penetrate the spore covering. You have to heat the spore to open it and let the stain in.
What are the properties that differentiate the spore from the vegetative cell?
In the spore stain malachite green is used as a primary stain. This is driven into the cell by heat because of the impermeability of the spore. The stain is allowed to sit for 30 mins to make sure it gets in to the endospores.The stain is then washed and counterstained with safranin red. The endospores retain the green colour from malachite green and of course appear green under the microscope. Whereas the vegetative cells will appear red.
What would you expect from a gram stain done on a slide that was heated too hot during the heat-fixed smear?
A Gram stain refers to a staining technique for the identification of bacteria. A Gram stain done on a slide that was heated too hot during the heat-fixed smear will destroy the cell wall of the bacteria.
What happens if you don't heat fix a smear prior to staining of a bacterial slide?
smear will be washed( no smear will be left on the slide)
Give the reason for passing bacterial smear through the flame before staining?
It dries the smear and fixes the cells to the slide
What mordant is used in spore staining?
Heat is the mordant used in the spore stain, it fixes the primary stain.
What is the purpose of staining the smear in malachite green during spore staining?
the purpose of boiling of smear in malachite green is to forces a stain to penetrate the endospore wall, it is necessary to heat the slide and the stain to prod the wall to allow the stain to enter.
Why gram staining procedure cannot stain endospores?
Because stains can't penetrate the spore covering. You have to heat the spore to open it and let the stain in.
Why is it essential to apply heat during endospore staining?
because they cannot be differentiated from inclusions of stored material without a special stain .
What would the results be if you used the TB decolorizer during the spore stain?
Depends if heat is used
In spore staining what would happen it you used safarin as the primary stain and malachite green as the counterstain?
The spore would appear to be red as the safranin is heat driven into the many layers of the spore, however, as Malachite green has a weak affinity and is water soluble, it will not likely bind to the spore wall or the cell wall. You might have traces of green on the slide if any, but it will be very little. Your vegetative cells will be pink as well.
How do you know when to do a spore stain?
endospores can't be stained by ordinary methods, such as simple and gram staining, because the stain can't penetrate the endospore's wall, therefore you must heat the stain to help it penetrate the wall
What are the properties that differentiate the spore from the vegetative cell?
In the spore stain malachite green is used as a primary stain. This is driven into the cell by heat because of the impermeability of the spore. The stain is allowed to sit for 30 mins to make sure it gets in to the endospores.The stain is then washed and counterstained with safranin red. The endospores retain the green colour from malachite green and of course appear green under the microscope. Whereas the vegetative cells will appear red.
Why is the size more accurate in a negative stain than a simple stain?
since you do not heat fix the slide when you use a negative stain the cells do not shrink or become distorted
What would you expect from a gram stain done on a slide that was heated too hot during the heat-fixed smear?
A Gram stain refers to a staining technique for the identification of bacteria. A Gram stain done on a slide that was heated too hot during the heat-fixed smear will destroy the cell wall of the bacteria.
What happens if you don't heat fix a smear prior to staining of a bacterial slide?
smear will be washed( no smear will be left on the slide)
Give the reason for passing bacterial smear through the flame before staining?
It dries the smear and fixes the cells to the slide
