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It means that the organism can use Lactose as an energy source
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What is mcConkey agar?
MacConkey agar is a selective medium designed to grow Gram negavite bacteria and stain them for lactose fermentation. It contains bile salts[to inhibit most Gram negative bacteria and some species of Staphlococcus], crystal violet dye, neutral red dye ,lactose and peptone. It was first developed by Alfred Theodore MacConkey. It is used to distinguish between lactose fermenting bacteria and non lactose fermenting bacteria. By utilising the lactose available in the medium, Lac+ bacteria such as Escherichia coli, Enterobacter and Klebsiella will produce acid, which lowers the pH of the agar below 6.8 and results in the appearance of red/pink colonies. Non-Lactose fermenting bacteria such as Salmonella, Proteus species and Shigella cannot utilize lactose, and will use peptone instead. This forms ammonia, which raises the pH of the agar, and leads to the formation of white/colorless colonies
Is lactic acid bacteria a bacteria?
Yes lactic acid fermenting bacteria is also a bacteria. It is rich in milk, yogurt. It ferments the lactose in the milk to lactic acid.
How much ethanol does fermenting 100g of sugar produce?
Ethanol produces around 80% by fermenting 100g of sugar produce.
What is difference between lactic acid and lactose?
If we are talking about distinguishing between the two solutions then, Carry out a Benedict's test on both solutions; Results: Lactose solution would give a brick-red/brown precipitate (positive test) Sucrose solution would give negative test result while sucrose is a non-reducing sugar which does not react with Benedict's reagent.
Which enzyme converts lactose to glucose and lactose?
Lactase is the enzyme that breaks down lactose.
What is negative lactose fermentative organisms?
these are those bacteria which are not able to ferment lactose sugar hence are called non lactose fermenting or negative lactose fermenting organisms.
Can shigella sonnei be a lactose non fermenter My tests are as followed TSI KA Methyl red VP - citrate - urea - NO3 degraded to NO2 H2S - motility -. all points to shigella but it's nonferm?
S.sonnei is not a true lactose fermenting bacteria (see.. http://www.pubmedcentral.nih.gov/picrender.fcgi?artid=271578&blobtype=pdf)But is a delayed lactose fermenter as it does not have the enzyme permease to transport lactose through its cell wall.However an ONPG lactose fermenting test does not require permease to allow the bacterium to ferment lactose so in an ONPG test the shigella sonnei will appear as lactose fermenting positive.Hope this helps!!2nd year BMS student
Why does the ecoli and pseudomonas are different on the MacConkey agar?
MacConkey's agar is a differential media used to differentiate between lactose fermenting and lactose non-fermenting bacteria. E.coli is a lactose fermenter whereas Pseudomonas is a lactose non-fermenter.MacConkey's agar contains lactose as fermentable sugar and when it is fermented the pH of the medium decreases which is registered by neutral red (a pH indicator).Lactose fermenters such as E.coli produce pink colonies whereas lactose non-fermenters such as Pseudomonas produces colorless colonies. So the colors of E.coli and Pseudomonas colonies are different on MacConkey's agar.
What is mcConkey agar?
MacConkey agar is a selective medium designed to grow Gram negavite bacteria and stain them for lactose fermentation. It contains bile salts[to inhibit most Gram negative bacteria and some species of Staphlococcus], crystal violet dye, neutral red dye ,lactose and peptone. It was first developed by Alfred Theodore MacConkey. It is used to distinguish between lactose fermenting bacteria and non lactose fermenting bacteria. By utilising the lactose available in the medium, Lac+ bacteria such as Escherichia coli, Enterobacter and Klebsiella will produce acid, which lowers the pH of the agar below 6.8 and results in the appearance of red/pink colonies. Non-Lactose fermenting bacteria such as Salmonella, Proteus species and Shigella cannot utilize lactose, and will use peptone instead. This forms ammonia, which raises the pH of the agar, and leads to the formation of white/colorless colonies
What does fermenting must mean?
must mean you have to do it
Is lactose in lactic acid?
No. Lactic acid is a different molecule than lactose. Molecular Formulas: Lactic Acid- C3H6O3 Lactose- C12H22O11 In addition, lactose is a sugar molecular while, as you may guess, lactic acid is an acid. When raw milk turns sour or becomes curdled, lactic acid bacteria are fermenting lactose into lactic acid.
Why is MacConkey agar inoculated with Urine specimen?
MacConkey agar medium differentiates between the lactose fermenting and nonlactose fermenting bacteria. Most of the bacterial commensals from our large intestine are lactose fermenting, Escherichia coli being one of them. When bacterial growths and colonies on MacConkey agar is pink coloured, then the organism is fermenting lactose from the medium, on the other hand, if transparent/transluscent, colourless growths and colonies appear on the medium, the bacterium does not ferment lactose. The flushing action of urine, make it sterile in an uninfected healthy person. In an event where the patient is suspected to have urinary tract infection (UTI), urine of patient is inoculated on MacConkey agar medium and incubated at 37 degree centigrade overnight. In most of the cases, UTI is caused by Escherichia coli that is lactose fermenting and produces pink coloured colonies on MacConkey agar medium. Although the urine is sterile, it may get contaminated by the organisms of skin microflora or by surrounding microbes due to improper collection of urine. In such cases, UTI is diagnosed by counting bacteria in urine sample. For significant bacteriuria (bacteria in urine), the count must be equal to or more than 1 lack bacteria per mL of urine. Answered by: M. F. Khan, Dept. of Microbiology, GMC, jagdalpur (CG)
On pandas bigger adventure what does fermenting barel mean?
it means alcohol
Is lactic acid bacteria a bacteria?
Yes lactic acid fermenting bacteria is also a bacteria. It is rich in milk, yogurt. It ferments the lactose in the milk to lactic acid.
Are cats lactose and tolerant?
I think you mean "lactose intolerant". Yes, some cats are lactose intolerant. However in rare cases, they are not.
Can the Japanese drink milk or are they lactose intolerant?
no it doesnt matter what race your from you can be lactose intolerant if you are japaneese but just because you are from japan that does not mean you have to be lactose intolerant
What are at least three way in which microorganism are beneficial to humans?
Fermenting sugars to make alcohol, metabolising lactose to produce lactic acid to make yoghurt, decomposing dead matter, bread making, cheese making
