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Does b.subtilis facultative anaerobes in thioglycollate broth?
No
How does thioglycollate work?
Thioglycollate broth is an enriched medium used to determine the oxygen requirements of microorganisms. The diffusion of oxygen from the top of the broth provides a range of concentrations.
How can you tell if thioglycollate medium is prepared correctly?
Thioglycollate medium is correctly prepared when a red/brown color appears at the top of the broth. This is beacuse of the Reazurin which is reactive to oxygen.
What type of media is used to demonstrate oxygen requirements of microbes?
thioglycollate
What does a blue or pink color in thioglycollate mean?
If its Fluid thioglycollate media - a red colour indicates thatg the media has been oxygenated - which is not desirable. The colour change is due to a chemical called resazurin in the media.
Predict the charge that an ion of sodium would have?
I predict that an ion of sodium will have a charge of plus one.
Why will obligate anaerobes grow in thioglycollate?
Obligate anaerobes do not need or use oxygen as a nutrient. In fact it is a toxic substance to them which either kills or inhibits their growth. Thioglycollate allows obligate anaerobes to grow because it consists of sodium thioglycollate, thioglycollic acid and L-crystine which reduce oxygen to water. This creates an anaerobic environment allowing obligate anaerobes to grow.
The properties and purposes of thioglycollate medium?
A thioglycollate medium is used for culturing anaerobic bacteria.
Predict the products resulting from the decomposition of sodium chlorate?
sodium chloride and oxygen gas.
Why is sodium thioglycollate used in lab media?
It is used as a reducing media. Thus minimizing the level of oxygen in the media.
What is the chemical composition of fluid thioglycollate medium?
Enzymatic Digest of Casein 15 g,Yeast Extract 5 g,Dextrose 5.5 g, L-Cystine 0.5 g,Sodium Chloride 2.5 g,Sodium Thioglycollate 0.5 g,Resazurin 0.001 g, Agar 0.75 g Final pH: 7.1 ± 0.2 at 25°C Formula may be adjusted and/or supplemented as required to meet performance specifications
Does b.subtilis facultative anaerobes in thioglycollate broth?
No
How does thioglycollate work?
Thioglycollate broth is an enriched medium used to determine the oxygen requirements of microorganisms. The diffusion of oxygen from the top of the broth provides a range of concentrations.
How can you tell if thioglycollate medium is prepared correctly?
Thioglycollate medium is correctly prepared when a red/brown color appears at the top of the broth. This is beacuse of the Reazurin which is reactive to oxygen.
Why are cysteine and sodium sulfide added to the growth media?
To culture obligate anaerobes, all molecular oxygen must be removed and kept out of the medium. Addition of oxygen-binding agents such as thioglycollate, the amino acid cysteine, or sodium sulfide to the medium prevents oxygen from killing the anaerobes you want to culture.
What is the function of thiogllycollic acid in thioglycollate?
It removes trapped oxygen from the medium
What does Thioglycollate contribute to culture media?
Look for this information in the link below.
