You mean heating the enzymes? heating to a certain degree increases their rate of catalytic activity, until about 30-40 degrees Celsius. However if you continue heating, the enzyme's basic structure will be deformed (it changes shape), and since structure is essential to function, the enzymes activity will be reduced. that happens around 60-70 degrees Celsius, but it is different in various organisms.
Enzymes serve as catalysts for reactions. they must come into contact with the reactants in order to form the product. At freezing temperatures, there is insufficient movement of the atoms and molecules to bump into each other and react. Enzyme activity will increase as temperature increases until a certain point when production drops off. Most enzymes are proteins which will denature at excessively high temperatures. Very few enzymes can function at boiling.
Denaturation of enzymes take place and they lose their activity.
The pH is varied to effect, by its affect, this test.
At low concentration of substrate , rate of enzyme action is directly proportional to conc. of substrate .
Different enzymes work best at different pH. This is refered to as the ideal pH for the enzyme. For example, the digestive enzyme trypsin works best at an acidic pH while alkaline phosphatase works best at a basic pH. Therefore, enzyme activity varies with pH and this variation depends on the enzyme being studied
activators; inhibitors
Phosphorylated enzymes may be more or less active than non-phosphorylated enzymes
Denature enzyme activity
Cold temperatures have a drastic effect on an enzyme's activity level. Cold temperatures usually dramatically slow down an enzyme's activity.
pH
The pH is varied to effect, by its affect, this test.
temperature and pH
At low concentration of substrate , rate of enzyme action is directly proportional to conc. of substrate .
many vitamins and minerals play crucial roles in many metabolisms as coenzyme or cofactor. Deficiency of those lower the related-enzyme activity.
The enzyme is inactive at this point. New enzyme must be added to regain enzyme activity
Physical activity can alter the shape of enzyme which can cause damage or may the enzyme become inactive
It would lower the speed of the reaction but will do no harm to the enzyme.
Different enzymes work best at different pH. This is refered to as the ideal pH for the enzyme. For example, the digestive enzyme trypsin works best at an acidic pH while alkaline phosphatase works best at a basic pH. Therefore, enzyme activity varies with pH and this variation depends on the enzyme being studied
effect of ph and concentration of subtrate