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Probably the Missouri Compromise of 1920. It started the debates about whether or not the new western states would allow slavery. It temporarily stopped the spread of slaver north of the 36 30 parallel, but would eventually lead to tensions between the North and the South and promote the Civil War.

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Q: What had the greatest impact on the institution of slavery in the US in the first quarter of the 19th century?
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What had the greatest impact on the institution of slavery in the US in the first quarter of the nineteenth century?

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